NLE1 Antibody
Catalog Number: LS-C676160
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NLE1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80716-20
Catalog Number: LS-G80716
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NLE1 (NM_018096) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NLE1 | Notchless gene homolog | Nle | Notchless protein homolog 1
- Origin Species
- Human
- NCBI GenBank
- NM_018096
- SwissProt
- Q9NVX2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAVADEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA LLAQEDPLPL61AFFVHDAEIV SSLGKTLESQ AVETEKVLDI IYQPQAIFRV RAVTRCTSSL EGHSEAVISV121AFSPTGKYLA SGSGDTTVRF WDLSTETPHF TCKGHRHWVL SISWSPDGKK LASGCKNGQI181LLWDPSTGKQ VGRTLAGHSK WITGLSWEPL HANPECRYVA SSSKDGSVRI WDTTAGRCER241ILTGHTQSVT CLRWGGDGLL YSASQDRTIK VWRAHDGVLC RTLQGHGHWV NTMALSTDYA301LRTGAFEPAE ASVNPQDLKG SLQELKERAL SRYNLVRGQG PERLVSGSDD FTLFLWSPAE361DKKPLTRMTG HQALINQVLF SPDSRIVASA SFDKSIKLWD GRTGKYLASL RGHVAAVYQI421AWSADSRLLV SGSSDSTLKV WDVKAQKLAM DLPGHADEVY AVDWSPDGQR VASGGKDKCL481RIWRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
485 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
