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NHSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NHSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NHSL1 antibodies →
$725.00each
Catalog Number: LS-G128467-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NHSL1 (NM_020464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf63 | KIAA1357 | NHS-like 1 | BA43P8.1 | NHS-like protein 1 | NHSL1
Origin Species
Human
NCBI GenBank
AB037778
SwissProt
Q5SYE7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
170.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKEGSSGSF RLQPNTGSLS RAVSWINFSS LSRQTKRLFR SDGELSVCGQ QVEVDDENWI
61YRAQPRKAVS NLDEESRWTV HYTAPWHQQE NVFLPTTRPP CVEDLHRQAK LNLKSVLREC
121DKLRHDGYRS SQYYSQGPTF AANASPFCDD YQDEDEETDQ KCSLSSSEEE RFISIRRPKT
181PASSDFSDLN TQTNWTKSLP LPTPEEKMRQ QAQTVQADVV PINITASGTG QDDADGHSVY
241TPDHYSTLGR FNSCRSAGQR SETRDSSCQT EDVKVVPPSM RRIRAQKGQG IAAQMGHFSG
301SSGNMSVLSD SAGIVFPSRL DSDAGFHSLP RSGARANIQS LEPRLGALGP AGDMNGTFLY
361QRGHPQADEN LGHLGGASGT GTLLRPKSQE LRHFESENIM SPACVVSPHA TYSTSIIPNA
421TLSSSSEVIA IPTAQSAGQR ESKSSGSSHA RIKSRDHLIS RHAVKGDPQS PGRHWNEGHA
481TILSQDLDPH SPGEPALLSL CDSAVPLNAP ANRENGSQAM PYNCRNNLAF PAHPQDVDGK
541SESSYSGGGG HSSSEPWEYK SSGNGRASPL KPHLATPGYS TPTSNMSSCS LDQTSNKEDA
601GSLYSEDHDG YCASVHTDSG HGSGNLCNSS DGFGNPRHSV INVFVGRAQK NQGDRSNYQD
661KSLSRNISLK KAKKPPLPPS RTDSLRRIPK KSSQCNGQVL NESLIATLQH SLQLSLPGKS
721GSSPSQSPCS DLEEPWLPRS RSQSTVSAGS SMTSATTPNV YSLCGATPSQ SDTSSVKSEY
781TDPWGYYIDY TGMQEDPGNP AGGCSTSSGV PTGNGPVRHV QEGSRATMPQ VPGGSVKPKI
841MSPEKSHRVI SPSSGYSSQS NTPTALTPVP VFLKSVSPAN GKGKPKPKVP ERKSSLISSV
901SISSSSTSLS SSTSTEGSGT MKKLDPAVGS PPAPPPPPVP SPPFPCPADR SPFLPPPPPV
961TDCSQGSPLP HSPVFPPPPP EALIPFCSPP DWCLSPPRPA LSPILPDSPV SLPLPPPLLP
1021SSEPPPAPPL DPKFMKDTRP PFTNSGQPES SRGSLRPPST KEETSRPPMP LITTEALQMV
1081QLRPVRKNSG AEAAQLSERT AQEQRTPVAP QYHLKPSAFL KSRNSTNEME SESQPASVTS
1141SLPTPAKSSS QGDHGSAAER GGPVSRSPGA PSAGEAEARP SPSTTPLPDS SPSRKPPPIS
1201KKPKLFLVVP PPQKDFAVEP AENVSEALRA VPSPTTGEEG SVHSREAKES SAAQAGSHAT
1261HPGTSVLEGG AAGSMSPSRV EANVPMVQPD VSPAPKQEEP AENSADTGGD GESCLSQQDG
1321AAGVPETNAA GSSSEACDFL KEDGNDEVMT PSRPRTTEDL FAAIHRSKRK VLGRRDSDDD
1381HSRNHSPSPP VTPTGAAPSL ASPKQVGSIQ RSIRKSSTSS DNFKALLLKK GSRSDTSARM
1441SAAEMLKNTD PRFQRSRSEP SPDAPESPSS CSPSKNRRAQ EEWAKNEGLM PRSLSFSGPR
1501YGRSRTPPSA ASSRYSMRNR IQSSPMTVIS EGEGEAVEPV DSIARGALGA AEGCSLDGLA
1561REEMDEGGLL CGEGPAASLQ PQAPGPVDGT ASAEGREPSP QCGGSLSEES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.