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Human NHSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NHSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NHSL1 antibodies →
$220.00each
Catalog Number: LS-G84628-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NHSL1 (NM_020464) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf63 | KIAA1357 | NHS-like 1 | BA43P8.1 | NHS-like protein 1 | NHSL1
Origin Species
Human
NCBI GenBank
AB037778
SwissProt
Q5SYE7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
170.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKEGSSGSF RLQPNTGSLS RAVSWINFSS LSRQTKRLFR SDGELSVCGQ QVEVDDENWI
61YRAQPRKAVS NLDEESRWTV HYTAPWHQQE NVFLPTTRPP CVEDLHRQAK LNLKSVLREC
121DKLRHDGYRS SQYYSQGPTF AANASPFCDD YQDEDEETDQ KCSLSSSEEE RFISIRRPKT
181PASSDFSDLN TQTNWTKSLP LPTPEEKMRQ QAQTVQADVV PINITASGTG QDDADGHSVY
241TPDHYSTLGR FNSCRSAGQR SETRDSSCQT EDVKVVPPSM RRIRAQKGQG IAAQMGHFSG
301SSGNMSVLSD SAGIVFPSRL DSDAGFHSLP RSGARANIQS LEPRLGALGP AGDMNGTFLY
361QRGHPQADEN LGHLGGASGT GTLLRPKSQE LRHFESENIM SPACVVSPHA TYSTSIIPNA
421TLSSSSEVIA IPTAQSAGQR ESKSSGSSHA RIKSRDHLIS RHAVKGDPQS PGRHWNEGHA
481TILSQDLDPH SPGEPALLSL CDSAVPLNAP ANRENGSQAM PYNCRNNLAF PAHPQDVDGK
541SESSYSGGGG HSSSEPWEYK SSGNGRASPL KPHLATPGYS TPTSNMSSCS LDQTSNKEDA
601GSLYSEDHDG YCASVHTDSG HGSGNLCNSS DGFGNPRHSV INVFVGRAQK NQGDRSNYQD
661KSLSRNISLK KAKKPPLPPS RTDSLRRIPK KSSQCNGQVL NESLIATLQH SLQLSLPGKS
721GSSPSQSPCS DLEEPWLPRS RSQSTVSAGS SMTSATTPNV YSLCGATPSQ SDTSSVKSEY
781TDPWGYYIDY TGMQEDPGNP AGGCSTSSGV PTGNGPVRHV QEGSRATMPQ VPGGSVKPKI
841MSPEKSHRVI SPSSGYSSQS NTPTALTPVP VFLKSVSPAN GKGKPKPKVP ERKSSLISSV
901SISSSSTSLS SSTSTEGSGT MKKLDPAVGS PPAPPPPPVP SPPFPCPADR SPFLPPPPPV
961TDCSQGSPLP HSPVFPPPPP EALIPFCSPP DWCLSPPRPA LSPILPDSPV SLPLPPPLLP
1021SSEPPPAPPL DPKFMKDTRP PFTNSGQPES SRGSLRPPST KEETSRPPMP LITTEALQMV
1081QLRPVRKNSG AEAAQLSERT AQEQRTPVAP QYHLKPSAFL KSRNSTNEME SESQPASVTS
1141SLPTPAKSSS QGDHGSAAER GGPVSRSPGA PSAGEAEARP SPSTTPLPDS SPSRKPPPIS
1201KKPKLFLVVP PPQKDFAVEP AENVSEALRA VPSPTTGEEG SVHSREAKES SAAQAGSHAT
1261HPGTSVLEGG AAGSMSPSRV EANVPMVQPD VSPAPKQEEP AENSADTGGD GESCLSQQDG
1321AAGVPETNAA GSSSEACDFL KEDGNDEVMT PSRPRTTEDL FAAIHRSKRK VLGRRDSDDD
1381HSRNHSPSPP VTPTGAAPSL ASPKQVGSIQ RSIRKSSTSS DNFKALLLKK GSRSDTSARM
1441SAAEMLKNTD PRFQRSRSEP SPDAPESPSS CSPSKNRRAQ EEWAKNEGLM PRSLSFSGPR
1501YGRSRTPPSA ASSRYSMRNR IQSSPMTVIS EGEGEAVEPV DSIARGALGA AEGCSLDGLA
1561REEMDEGGLL CGEGPAASLQ PQAPGPVDGT ASAEGREPSP QCGGSLSEES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.