Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NGN / Neogenin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99774-100
Catalog Number: LS-G99774
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neogenin(NEO1) (NM_002499), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Neogenin homolog 1 | Neogenin (chicken) homolog 1 | NEO1 | HsT17534 | IGDCC2 | Neogenin | Neogenin 1 | NGN
- NCBI GenBank
- NM_002499
- SwissProt
- Q92859
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 160.02 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAERGARRL LSTPSFWLYC LLLLGRRAPG AAAARSGSAP QSPGASIRTF TPFYFLVEPV61DTLSVRGSSV ILNCSAYSEP SPKIEWKKDG TFLNLVSDDR RQLLPDGSLF ISNVVHSKHN121KPDEGYYQCV ATVESLGTII SRTAKLIVAG LPRFTSQPEP SSVYAGNNAI LNCEVNADLV181PFVRWEQNRQ PLLLDDRVIK LPSGMLVISN ATEGDGGLYR CVVESGGPPK YSDEVELKVL241PDPEVISDLV FLKQPSPLVR VIGQDVVLPC VASGLPTPTI KWMKNEEALD TESSERLVLL301AGGSLEISDV TEDDAGTYFC IADNGNETIE AQAELTVQAQ PEFLKQPTNI YAHESMDIVF361ECEVTGKPTP TVKWVKNGDM VIPSDYFKIV KEHNLQVLGL VKSDEGFYQC IAENDVGNAQ421AGAQLIILEH APATTGPLPS APRDVVASLV STRFIKLTWR TPASDPHGDN LTYSVFYTKE481GIARERVENT SHPGEMQVTI QNLMPATVYI FRVMAQNKHG SGESSAPLRV ETQPEVQLPG541PAPNLRAYAA SPTSITVTWE TPVSGNGEIQ NYKLYYMEKG TDKEQDVDVS SHSYTINGLK601KYTEYSFRVV AYNKHGPGVS TPDVAVRTLS DVPSAAPQNL SLEVRNSKSI MIHWQPPAPA661TQNGQITGYK IRYRKASRKS DVTETLVSGT QLSQLIEGLD RGTEYNFRVA ALTINGTGPA721TDWLSAETFE SDLDETRVPE VPSSLHVRPL VTSIVVSWTP PENQNIVVRG YAIGYGIGSP781HAQTIKVDYK QRYYTIENLD PSSHYVITLK AFNNVGEGIP LYESAVTRPH TDTSEVDLFV841INAPYTPVPD PTPMMPPVGV QASILSHDTI RITWADNSLP KHQKITDSRY YTVRWKTNIP901ANTKYKNANA TTLSYLVTGL KPNTLYEFSV MVTKGRRSST WSMTAHGTTF ELVPTSPPKD961VTVVSKEGKP KTIIVNWQPP SEANGKITGY IIYYSTDVNA EIHDWVIEPV VGNRLTHQIQ1021ELTLDTPYYF KIQARNSKGM GPMSEAVQFR TPKADSSDKM PNDQASGSGG KGSRLPDLGS1081DYKPPMSGSN SPHGSPTSPL DSNMLLVIIV SVGVITIVVV VIIAVFCTRR TTSHQKKKRA1141ACKSVNGSHK YKGNSKDVKP PDLWIHHERL ELKPIDKSPD PNPIMTDTPI PRNSQDITPV1201DNSMDSNIHQ RRNSYRGHES EDSMSTLAGR RGMRPKMMMP FDSQPPQPVI SAHPIHSLDN1261PHHHFHSSSL ASPARSHLYH PGSPWPIGTS MSLSDRANST ESVRNTPSTD TMPASSSQTC1321CTDHQDPEGA TSSSYLASSQ EEDSGQSLPT AHVRPSHPLK SFAVPAIPPP GPPTYDPALP1381STPLLSQQAL NHHIHSVKTA SIGTLGRSRP PMPVVVPSAP EVQETTRMLE DSESSYEPDE1441LTKEMAHLEG LMKDLNAITT ATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1461 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.