LSBio
Human NGN / Neogenin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NGN / Neogenin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NEO1 antibodies →
$220.00each
Catalog Number: LS-G81685-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Neogenin(NEO1) (NM_002499) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neogenin homolog 1 | Neogenin (chicken) homolog 1 | NEO1 | HsT17534 | IGDCC2 | Neogenin | Neogenin 1 | NGN
Origin Species
Human
NCBI GenBank
NM_002499
SwissProt
Q92859

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
160.02 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAERGARRL LSTPSFWLYC LLLLGRRAPG AAAARSGSAP QSPGASIRTF TPFYFLVEPV
61DTLSVRGSSV ILNCSAYSEP SPKIEWKKDG TFLNLVSDDR RQLLPDGSLF ISNVVHSKHN
121KPDEGYYQCV ATVESLGTII SRTAKLIVAG LPRFTSQPEP SSVYAGNNAI LNCEVNADLV
181PFVRWEQNRQ PLLLDDRVIK LPSGMLVISN ATEGDGGLYR CVVESGGPPK YSDEVELKVL
241PDPEVISDLV FLKQPSPLVR VIGQDVVLPC VASGLPTPTI KWMKNEEALD TESSERLVLL
301AGGSLEISDV TEDDAGTYFC IADNGNETIE AQAELTVQAQ PEFLKQPTNI YAHESMDIVF
361ECEVTGKPTP TVKWVKNGDM VIPSDYFKIV KEHNLQVLGL VKSDEGFYQC IAENDVGNAQ
421AGAQLIILEH APATTGPLPS APRDVVASLV STRFIKLTWR TPASDPHGDN LTYSVFYTKE
481GIARERVENT SHPGEMQVTI QNLMPATVYI FRVMAQNKHG SGESSAPLRV ETQPEVQLPG
541PAPNLRAYAA SPTSITVTWE TPVSGNGEIQ NYKLYYMEKG TDKEQDVDVS SHSYTINGLK
601KYTEYSFRVV AYNKHGPGVS TPDVAVRTLS DVPSAAPQNL SLEVRNSKSI MIHWQPPAPA
661TQNGQITGYK IRYRKASRKS DVTETLVSGT QLSQLIEGLD RGTEYNFRVA ALTINGTGPA
721TDWLSAETFE SDLDETRVPE VPSSLHVRPL VTSIVVSWTP PENQNIVVRG YAIGYGIGSP
781HAQTIKVDYK QRYYTIENLD PSSHYVITLK AFNNVGEGIP LYESAVTRPH TDTSEVDLFV
841INAPYTPVPD PTPMMPPVGV QASILSHDTI RITWADNSLP KHQKITDSRY YTVRWKTNIP
901ANTKYKNANA TTLSYLVTGL KPNTLYEFSV MVTKGRRSST WSMTAHGTTF ELVPTSPPKD
961VTVVSKEGKP KTIIVNWQPP SEANGKITGY IIYYSTDVNA EIHDWVIEPV VGNRLTHQIQ
1021ELTLDTPYYF KIQARNSKGM GPMSEAVQFR TPKADSSDKM PNDQASGSGG KGSRLPDLGS
1081DYKPPMSGSN SPHGSPTSPL DSNMLLVIIV SVGVITIVVV VIIAVFCTRR TTSHQKKKRA
1141ACKSVNGSHK YKGNSKDVKP PDLWIHHERL ELKPIDKSPD PNPIMTDTPI PRNSQDITPV
1201DNSMDSNIHQ RRNSYRGHES EDSMSTLAGR RGMRPKMMMP FDSQPPQPVI SAHPIHSLDN
1261PHHHFHSSSL ASPARSHLYH PGSPWPIGTS MSLSDRANST ESVRNTPSTD TMPASSSQTC
1321CTDHQDPEGA TSSSYLASSQ EEDSGQSLPT AHVRPSHPLK SFAVPAIPPP GPPTYDPALP
1381STPLLSQQAL NHHIHSVKTA SIGTLGRSRP PMPVVVPSAP EVQETTRMLE DSESSYEPDE
1441LTKEMAHLEG LMKDLNAITT A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1461 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.