Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFKB1 / NF-Kappa-B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116921-100
Catalog Number: LS-G116921
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFKB1(NFKB1) (NM_003998), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta | NFKB1
- Origin Species
- Human
- NCBI GenBank
- NM_003998
- SwissProt
- P19838
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEDDPYLGR PEQMFHLDPS LTHTIFNPEV FQPQMALPTA DGPYLQILEQ PKQRGFRFRY61VCEGPSHGGL PGASSEKNKK SYPQVKICNY VGPAKVIVQL VTNGKNIHLH AHSLVGKHCE121DGICTVTAGP KDMVVGFANL GILHVTKKKV FETLEARMTE ACIRGYNPGL LVHPDLAYLQ181AEGGGDRQLG DREKELIRQA ALQQTKEMDL SVVRLMFTAF LPDSTGSFTR RLEPVVSDAI241YDSKAPNASN LKIVRMDRTA GCVTGGEEIY LLCDKVQKDD IQIRFYEEEE NGGVWEGFGD301FSPTDVHRQF AIVFKTPKYK DINITKPASV FVQLRRKSDL ETSEPKPFLY YPEIKDKEEV361QRKRQKLMPN FSDSFGGGSG AGAGGGGMFG SGGGGGGTGS TGPGYSFPHY GFPTYGGITF421HPGTTKSNAG MKHGTMDTES KKDPEGCDKS DDKNTVNLFG KVIETTEQDQ EPSEATVGNG481EVTLTYATGT KEESAGVQDN LFLEKAMQLA KRHANALFDY AVTGDVKMLL AVQRHLTAVQ541DENGDSVLHL AIIHLHSQLV RDLLEVTSGL ISDDIINMRN DLYQTPLHLA VITKQEDVVE601DLLRAGADLS LLDRLGNSVL HLAAKEGHDK VLSILLKHKK AALLLDHPNG DGLNAIHLAM661MSNSLPCLLL LVAAGADVNA QEQKSGRTAL HLAVEHDNIS LAGCLLLEGD AHVDSTTYDG721TTPLHIAAGR GSTRLAALLK AAGADPLVEN FEPLYDLDDS WENAGEDEGV VPGTTPLDMA781TSWQVFDILN GKPYEPEFTS DDLLAQGDMK QLAEDVKLQL YKLLEIPDPD KNWATLAQKL841GLGILNNAFR LSPAPSKTLM DNYEVSGGTV RELVEALRQM GYTEAIEVIQ AASSPVKTTS901QAHSLPLSPA STRQQIDELR DSDSVCDSGV ETSFRKLSFT ESLTSGASLL TLNKMPHDYG961QEGPLEGKITRTRPLEQKLISEEDLAANDILDYKDDDDKV
969 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.