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NFAT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NFAT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124258-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFAT5 (NM_001113178), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NFAT-like protein 1 | NFATZ | OREBP | KIAA0827 | TonE-binding protein | TONEBP | NFAT5 | Glutamine rich protein H65 | NF-AT5 | NFATL1
Origin Species
Human
NCBI GenBank
NM_006599
SwissProt
O94916

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
167.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSDFISLLS ADLDLESPKS LYSRDSLKLH PSQNFHRAGL LEESVYDLLP KELQLPPSRE
61TSVASMSQTS GGEAGSPPPA VVAADASSAP SSSSMGGACS SFTTSSSPTI YSTSVTDSKA
121MQVESCSSAV GVSNRGVSEK QLTSNTVQQH PSTPKRHTVL YISPPPEDLL DNSRMSCQDE
181GCGLESEQSC SMWMEDSPSN FSNMSTSSYN DNTEVPRKSR KRNPKQRPGV KRRDCEESNM
241DIFDADSAKA PHYVLSQLTT DNKGNSKAGN GTLENQKGTG VKKSPMLCGQ YPVKSEGKEL
301KIVVQPETQH RARYLTEGSR GSVKDRTQQG FPTVKLEGHN EPVVLQVFVG NDSGRVKPHG
361FYQACRVTGR NTTPCKEVDI EGTTVIEVGL DPSNNMTLAV DCVGILKLRN ADVEARIGIA
421GSKKKSTRAR LVFRVNIMRK DGSTLTLQTP SSPILCTQPA GVPEILKKSL HSCSVKGEEE
481VFLIGKNFLK GTKVIFQENV SDENSWKSEA EIDMELFHQN HLIVKVPPYH DQHITLPVSV
541GIYVVTNAGR SHDVQPFTYT PDPAAGALNV NVKKEISSPA RPCSFEEAMK AMKTTGCNLD
601KVNIIPNALM TPLIPSSMIK SEDVTPMEVT AEKRSSTIFK TTKSVGSTQQ TLENISNIAG
661NGSFSSPSSS HLPSENEKQQ QIQPKAYNPE TLTTIQTQDI SQPGTFPAVS ASSQLPNSDA
721LLQQATQFQT RETQSREILQ SDGTVVNLSQ LTEASQQQQQ SPLQEQAQTL QQQISSNIFP
781SPNSVSQLQN TIQQLQAGSF TGSTASGSSG SVDLVQQVLE AQQQLSSVLF SAPDGNENVQ
841EQLSADIFQQ VSQIQSGVSP GMFSSTEPTV HTRPDNLLPG RAESVHPQSE NTLSNQQQQQ
901QQQQQVMESS AAMVMEMQQS ICQAAAQIQS ELFPSTASAN GNLQQSPVYQ QTSHMMSALS
961TNEDMQMQCE LFSSPPAVSG NETSTTTTQQ VATPGTTMFQ TSSSGDGEET GTQAKQIQNS
1021VFQTMVQMQH SGDNQPQVNL FSSTKSMMSV QNSGTQQQGN GLFQQGNEMM SLQSGNFLQQ
1081SSHSQAQLFH PQNPIADAQN LSQETQGSLF HSPNPIVHSQ TSTTSSEQMQ PPMFHSQSTI
1141AVLQGSSVPQ DQQSTNIFLS QSPMNNLQTN TVAQEAFFAA PNSISPLQST SNSEQQAAFQ
1201QQAPISHIQT PMLSQEQAQP PQQGLFQPQV ALGSLPPNPM PQSQQGTMFQ SQHSIVAMQS
1261NSPSQEQQQQ QQQQQQQQQQ QQQSILFSNQ NTMATMASPK QPPPNMIFNP NQNPMANQEQ
1321QNQSIFHQQS NMAPMNQEQQ PMQFQSQSTV SSLQNPGPTQ SESSQTPLFH SSPQIQLVQG
1381SPSSQEQQVT LFLSPASMSA LQTSINQQDM QQSPLYSPQN NMPGIQGATS SPQPQATLFH
1441NTAGGTMNQL QNSPGSSQQT SGMFLFGIQN NCSQLLTSGP ATLPDQLMAI SQPGQPQNEG
1501QPPVTTLLSQ QMPENSPLAS SINTNQNIEK IDLLVSLQNQ GNNLTGSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1548 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.