NFAT5 Antibody
Catalog Number: LS-C813913
3 sizes / from $301.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NFAT5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89524-20
Catalog Number: LS-G89524
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NFAT5 (NM_001113178) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NFAT-like protein 1 | NFATZ | OREBP | KIAA0827 | TonE-binding protein | TONEBP | NFAT5 | Glutamine rich protein H65 | NF-AT5 | NFATL1
- Origin Species
- Human
- NCBI GenBank
- NM_006599
- SwissProt
- O94916
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 167.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSDFISLLS ADLDLESPKS LYSRDSLKLH PSQNFHRAGL LEESVYDLLP KELQLPPSRE61TSVASMSQTS GGEAGSPPPA VVAADASSAP SSSSMGGACS SFTTSSSPTI YSTSVTDSKA121MQVESCSSAV GVSNRGVSEK QLTSNTVQQH PSTPKRHTVL YISPPPEDLL DNSRMSCQDE181GCGLESEQSC SMWMEDSPSN FSNMSTSSYN DNTEVPRKSR KRNPKQRPGV KRRDCEESNM241DIFDADSAKA PHYVLSQLTT DNKGNSKAGN GTLENQKGTG VKKSPMLCGQ YPVKSEGKEL301KIVVQPETQH RARYLTEGSR GSVKDRTQQG FPTVKLEGHN EPVVLQVFVG NDSGRVKPHG361FYQACRVTGR NTTPCKEVDI EGTTVIEVGL DPSNNMTLAV DCVGILKLRN ADVEARIGIA421GSKKKSTRAR LVFRVNIMRK DGSTLTLQTP SSPILCTQPA GVPEILKKSL HSCSVKGEEE481VFLIGKNFLK GTKVIFQENV SDENSWKSEA EIDMELFHQN HLIVKVPPYH DQHITLPVSV541GIYVVTNAGR SHDVQPFTYT PDPAAGALNV NVKKEISSPA RPCSFEEAMK AMKTTGCNLD601KVNIIPNALM TPLIPSSMIK SEDVTPMEVT AEKRSSTIFK TTKSVGSTQQ TLENISNIAG661NGSFSSPSSS HLPSENEKQQ QIQPKAYNPE TLTTIQTQDI SQPGTFPAVS ASSQLPNSDA721LLQQATQFQT RETQSREILQ SDGTVVNLSQ LTEASQQQQQ SPLQEQAQTL QQQISSNIFP781SPNSVSQLQN TIQQLQAGSF TGSTASGSSG SVDLVQQVLE AQQQLSSVLF SAPDGNENVQ841EQLSADIFQQ VSQIQSGVSP GMFSSTEPTV HTRPDNLLPG RAESVHPQSE NTLSNQQQQQ901QQQQQVMESS AAMVMEMQQS ICQAAAQIQS ELFPSTASAN GNLQQSPVYQ QTSHMMSALS961TNEDMQMQCE LFSSPPAVSG NETSTTTTQQ VATPGTTMFQ TSSSGDGEET GTQAKQIQNS1021VFQTMVQMQH SGDNQPQVNL FSSTKSMMSV QNSGTQQQGN GLFQQGNEMM SLQSGNFLQQ1081SSHSQAQLFH PQNPIADAQN LSQETQGSLF HSPNPIVHSQ TSTTSSEQMQ PPMFHSQSTI1141AVLQGSSVPQ DQQSTNIFLS QSPMNNLQTN TVAQEAFFAA PNSISPLQST SNSEQQAAFQ1201QQAPISHIQT PMLSQEQAQP PQQGLFQPQV ALGSLPPNPM PQSQQGTMFQ SQHSIVAMQS1261NSPSQEQQQQ QQQQQQQQQQ QQQSILFSNQ NTMATMASPK QPPPNMIFNP NQNPMANQEQ1321QNQSIFHQQS NMAPMNQEQQ PMQFQSQSTV SSLQNPGPTQ SESSQTPLFH SSPQIQLVQG1381SPSSQEQQVT LFLSPASMSA LQTSINQQDM QQSPLYSPQN NMPGIQGATS SPQPQATLFH1441NTAGGTMNQL QNSPGSSQQT SGMFLFGIQN NCSQLLTSGP ATLPDQLMAI SQPGQPQNEG1501QPPVTTLLSQ QMPENSPLAS SINTNQNIEK IDLLVSLQNQ GNNLTGSFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1548 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
