Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Neurofascin / NF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128429-100
Catalog Number: LS-G128429
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neurofascin(NFASC) (NM_001160331), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Neurofascin homolog | NF | KIAA0756 | Neurofascin | Neurofascin homolog (chicken) | NFASC | NRCAML
- Origin Species
- Human
- NCBI GenBank
- AB018299
- SwissProt
- O94856
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARQPPPPWV HAAFLLCLLS LGGAIEIPMD LTQPPTITKQ SAKDHIVDPR DNILIECEAK61GNPAPSFHWT RNSRFFNIAK DPRVSMRRRS GTLVIDFRSG GRPEEYEGEY QCFARNKFGT121ALSNRIRLQV SKSPLWPKEN LDPVVVQEGA PLTLQCNPPP GLPSPVIFWM SSSMEPITQD181KRVSQGHNGD LYFSNVMLQD MQTDYSCNAR FHFTHTIQQK NPFTLKVLTN HPYNDSSLRN241HPDMYSARGV AERTPSFMYP QGTASSQMVL RGMDLLLECI ASGVPTPDIA WYKKGGDLPS301DKAKFENFNK ALRITNVSEE DSGEYFCLAS NKMGSIRHTI SVRVKAAPYW LDEPKNLILA361PGEDGRLVCR ANGNPKPTVQ WMVNGEPLQS APPNPNREVA GDTIIFRDTQ ISSRAVYQCN421TSNEHGYLLA NAFVSVLDVP PRMLSPRNQL IRVILYNRTR LDCPFFGSPI PTLRWFKNGQ481GSNLDGGNYH VYENGSLEIK MIRKEDQGIY TCVATNILGK AENQVRLEVK DPTRIYRMPE541DQVARRGTTV QLECRVKHDP SLKLTVSWLK DDEPLYIGNR MKKEDDSLTI FGVAERDQGS601YTCVASTELD QDLAKAYLTV LADQATPTNR LAALPKGRPD RPRDLELTDL AERSVRLTWI661PGDANNSPIT DYVVQFEEDQ FQPGVWHDHS KYPGSVNSAV LRLSPYVNYQ FRVIAINEVG721SSHPSLPSER YRTSGAPPES NPGDVKGEGT RKNNMEITWT PMNATSAFGP NLRYIVKWRR781RETREAWNNV TVWGSRYVVG QTPVYVPYEI RVQAENDFGK GPEPESVIGY SGEDYPRAAP841TEVKVRVMNS TAISLQWNRV YSDTVQGQLR EYRAYYWRES SLLKNLWVSQ KRQQASFPGD901RLRGVVSRLF PYSNYKLEMV VVNGRGDGPR SETKEFTTPE GVPSAPRRFR VRQPNLETIN961LEWDHPEHPN GIMIGYTLKY VAFNGTKVGK QIVENFSPNQ TKFTVQRTDP VSRYRFTLSA1021RTQVGSGEAV TEESPAPPNE ATPTAAYTNN QADIATQGWF IGLMCAIALL VLILLIVCFI1081KRSRGGKYPV REKKDVPLGP EDPKEEDGSF DYSDEDNKPL QGSQTSLDGT IKQQESDDSL1141VDYGEGGEGQ FNEDGSFIGQ YTVKKDKEET EGNESSEATS PVNAIYSLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1189 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.