Neurofascin / NF Antibody
Catalog Number: LS-C668640
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human Neurofascin / NF Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G88264-20
Catalog Number: LS-G88264
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Neurofascin(NFASC) (NM_001160331) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Neurofascin homolog | NF | KIAA0756 | Neurofascin | Neurofascin homolog (chicken) | NFASC | NRCAML
- Origin Species
- Human
- NCBI GenBank
- AB018299
- SwissProt
- O94856
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARQPPPPWV HAAFLLCLLS LGGAIEIPMD LTQPPTITKQ SAKDHIVDPR DNILIECEAK61GNPAPSFHWT RNSRFFNIAK DPRVSMRRRS GTLVIDFRSG GRPEEYEGEY QCFARNKFGT121ALSNRIRLQV SKSPLWPKEN LDPVVVQEGA PLTLQCNPPP GLPSPVIFWM SSSMEPITQD181KRVSQGHNGD LYFSNVMLQD MQTDYSCNAR FHFTHTIQQK NPFTLKVLTN HPYNDSSLRN241HPDMYSARGV AERTPSFMYP QGTASSQMVL RGMDLLLECI ASGVPTPDIA WYKKGGDLPS301DKAKFENFNK ALRITNVSEE DSGEYFCLAS NKMGSIRHTI SVRVKAAPYW LDEPKNLILA361PGEDGRLVCR ANGNPKPTVQ WMVNGEPLQS APPNPNREVA GDTIIFRDTQ ISSRAVYQCN421TSNEHGYLLA NAFVSVLDVP PRMLSPRNQL IRVILYNRTR LDCPFFGSPI PTLRWFKNGQ481GSNLDGGNYH VYENGSLEIK MIRKEDQGIY TCVATNILGK AENQVRLEVK DPTRIYRMPE541DQVARRGTTV QLECRVKHDP SLKLTVSWLK DDEPLYIGNR MKKEDDSLTI FGVAERDQGS601YTCVASTELD QDLAKAYLTV LADQATPTNR LAALPKGRPD RPRDLELTDL AERSVRLTWI661PGDANNSPIT DYVVQFEEDQ FQPGVWHDHS KYPGSVNSAV LRLSPYVNYQ FRVIAINEVG721SSHPSLPSER YRTSGAPPES NPGDVKGEGT RKNNMEITWT PMNATSAFGP NLRYIVKWRR781RETREAWNNV TVWGSRYVVG QTPVYVPYEI RVQAENDFGK GPEPESVIGY SGEDYPRAAP841TEVKVRVMNS TAISLQWNRV YSDTVQGQLR EYRAYYWRES SLLKNLWVSQ KRQQASFPGD901RLRGVVSRLF PYSNYKLEMV VVNGRGDGPR SETKEFTTPE GVPSAPRRFR VRQPNLETIN961LEWDHPEHPN GIMIGYTLKY VAFNGTKVGK QIVENFSPNQ TKFTVQRTDP VSRYRFTLSA1021RTQVGSGEAV TEESPAPPNE ATPTAAYTNN QADIATQGWF IGLMCAIALL VLILLIVCFI1081KRSRGGKYPV REKKDVPLGP EDPKEEDGSF DYSDEDNKPL QGSQTSLDGT IKQQESDDSL1141VDYGEGGEGQ FNEDGSFIGQ YTVKKDKEET EGNESSEATS PVNAIYSLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1189 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
