Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NET1 / ARHGEF8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119374-100
Catalog Number: LS-G119374
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NET1 (NM_001047160), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF8 | NET1A | NET1 | Proto-oncogene p65 Net1
- Origin Species
- Human
- NCBI GenBank
- NM_005863
- SwissProt
- Q7Z628
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPELAAQKQ PRPRRRSRRA SGLSTEGATG PSADTSGSEL DGRCSLRRGS SFTFLTPGPN61WDFTLKRKRR EKDDDVVSLS SLDLKEPSNK RVRPLARVTS LANLISPVRN GAVRRFGQTI121QSFTLRGDHR SPASAQKFSS RSTVPTPAKR RSSALWSEML DITMKESLTT REIRRQEAIY181EMSRGEQDLI EDLKLARKAY HDPMLKLSIM SEEELTHIFG DLDSYIPLHE DLLTRIGEAT241KPDGTVEQIG HILVSWLPRL NAYRGYCSNQ LAAKALLDQK KQDPRVQDFL QRCLESPFSR301KLDLWSFLDI PRSRLVKYPL LLKEILKHTP KEHPDVQLLE DAILIIQGVL SDINLKKGES361ECQYYIDKLE YLDEKQRDPR IEASKVLLCH GELRSKSGHK LYIFLFQDIL VLTRPVTRNE421RHSYQVYRQP IPVQELVLED LQDGDVRMGG SFRGAFSNSE KAKNIFRIRF HDPSPAQSHT481LQANDVFHKQ QWFNCIRAAI APFQSAGSPP ELQGLPELHE ECEGNHPSAR KLTAQRRAST541VSSVTQVEVD ENAYRCGSGM QMAEDSKSLK THQTQPGIRR ARDKALSGGK RKETLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
596 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.