NET1 / ARHGEF8 Antibody
Catalog Number: LS-C318233
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NET1 / ARHGEF8 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81403-20
Catalog Number: LS-G81403
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NET1 (NM_005863) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF8 | NET1A | NET1 | Proto-oncogene p65 Net1
- Origin Species
- Human
- NCBI GenBank
- NM_005863
- SwissProt
- Q7Z628
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAHDETGGL LPIKRTIRVL DVNNQSFREQ EEPSNKRVRP LARVTSLANL ISPVRNGAVR61RFGQTIQSFT LRGDHRSPAS AQKFSSRSTV PTPAKRRSSA LWSEMLDITM KESLTTREIR121RQEAIYEMSR GEQDLIEDLK LARKAYHDPM LKLSIMSEEE LTHIFGDLDS YIPLHEDLLT181RIGEATKPDG TVEQIGHILV SWLPRLNAYR GYCSNQLAAK ALLDQKKQDP RVQDFLQRCL241ESPFSRKLDL WSFLDIPRSR LVKYPLLLKE ILKHTPKEHP DVQLLEDAIL IIQGVLSDIN301LKKGESECQY YIDKLEYLDE KQRDPRIEAS KVLLCHGELR SKSGHKLYIF LFQDILVLTR361PVTRNERHSY QVYRQPIPVQ ELVLEDLQDG DVRMGGSFRG AFSNSEKAKN IFRIRFHDPS421PAQSHTLQAN DVFHKQQWFN CIRAAIAPFQ SAGSPPELQG LPELHEECEG NHPSARKLTA481QRRASTVSSV TQVEVDENAY RCGSGMQMAE DSKSLKTHQT QPGIRRARDK ALSGGKRKET541LVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
542 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
