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NEK5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NEK5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NEK5 antibodies →
$504.00each
Catalog Number: LS-G103378-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEK5 (NM_199289), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NIMA-related kinase 5 | NEK5 | NimA-related protein kinase 5
Origin Species
Human
NCBI GenBank
AX166554

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDKYDVIKAI GQGAFGKAYL AKGKSDSKHC VIKEINFEKM PIQEKEASKK EVILLEKMKH
61PNIVAFFNSF QENGRLFIVM EYCDGGDLMK RINRQRGVLF SEDQILGWFV QISLGLKHIH
121DRKILHRDIK AQNIFLSKNG MVAKLGDFGI ARVLNNSMEL ARTCIGTPYY LSPEICQNKP
181YNNKTDIWSL GCVLYELCTL KHPFEGNNLQ QLVLKICQAH FAPISPGFSR ELHSLISQLF
241QVSPRDRPSI NSILKRPFLE NLIPKYLTPE VIQEEFSHML ICRAGAPASR HAGKVVQKCK
301IQKVRFQGKC PPRSRISVPI KRNAILHRNE WRPPAGAQKA RSIKMIERPK IAAVCGHYDY
361YYAQLDMLRR RAHKPSYHPI PQENTGVEDY GQETRHGPSP SQWPAEYLQR KFEAQQYKLK
421VEKQLGLRPS SAEPNYNQRQ ELRSNGEEPR FQELPFRKNE MKEQEYWKQL EEIRQQYHND
481MKEIRKKMGR EPEENSKISH KTYLVKKSNL PVHQDASEGE APVQMEFRSC CPGWSAMARS
541WLTATSASQD IEKDLKQMRL QNTKESKNPE QKYKAKKGVK FEINLDKCIS DENILQEEEA
601MDIPNETLTF EDGMKFKEYE CVKEHGDYTD KAFEKLHCPE AGFSTQTVAA VGNRRQWDGG
661APQTLLQMMA VADITSTCPT GPDSESVLSV SRQEGKTKDP YSPVLILM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

708 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.