NEK5 Antibody
Catalog Number: LS-C402939
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NEK5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92233-20
Catalog Number: LS-G92233
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NEK5 (NM_199289) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NIMA-related kinase 5 | NEK5 | NimA-related protein kinase 5
- Origin Species
- Human
- NCBI GenBank
- AX166554
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDKYDVIKAI GQGAFGKAYL AKGKSDSKHC VIKEINFEKM PIQEKEASKK EVILLEKMKH61PNIVAFFNSF QENGRLFIVM EYCDGGDLMK RINRQRGVLF SEDQILGWFV QISLGLKHIH121DRKILHRDIK AQNIFLSKNG MVAKLGDFGI ARVLNNSMEL ARTCIGTPYY LSPEICQNKP181YNNKTDIWSL GCVLYELCTL KHPFEGNNLQ QLVLKICQAH FAPISPGFSR ELHSLISQLF241QVSPRDRPSI NSILKRPFLE NLIPKYLTPE VIQEEFSHML ICRAGAPASR HAGKVVQKCK301IQKVRFQGKC PPRSRISVPI KRNAILHRNE WRPPAGAQKA RSIKMIERPK IAAVCGHYDY361YYAQLDMLRR RAHKPSYHPI PQENTGVEDY GQETRHGPSP SQWPAEYLQR KFEAQQYKLK421VEKQLGLRPS SAEPNYNQRQ ELRSNGEEPR FQELPFRKNE MKEQEYWKQL EEIRQQYHND481MKEIRKKMGR EPEENSKISH KTYLVKKSNL PVHQDASEGE APVQMEFRSC CPGWSAMARS541WLTATSASQD IEKDLKQMRL QNTKESKNPE QKYKAKKGVK FEINLDKCIS DENILQEEEA601MDIPNETLTF EDGMKFKEYE CVKEHGDYTD KAFEKLHCPE AGFSTQTVAA VGNRRQWDGG661APQTLLQMMA VADITSTCPT GPDSESVLSV SRQEGKTKDP YSPVLILMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
708 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
