Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NCKIPSD / AF3P21 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103976-100
Catalog Number: LS-G103976
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCKIPSD (NM_184231), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AF3P21 | Dia-interacting protein 1 | DIP-1 | DIP1 | Dia interacting protein | DIP | NCKIPSD | ORF1 | SH3 adapter protein SPIN90 | SPIN90 | WISH | WASLBP | VIP54
- Origin Species
- Human
- NCBI GenBank
- NM_016453
- SwissProt
- Q9NZQ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYRALYAFRS AEPNALAFAA GETFLVLERS SAHWWLAARA RSGETGYVPP AYLRRLQGLE61QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGPS ASSVAVMTSS121TSDHHLDAAA ARQPNGVCRA GFERQHSLPS SEHLGADGGL YQIPPQPRRA APTTPPPPVK181RRDREALMAS GSGGHNTMPS GGNSVSSGSS VSSTSLDTLY TSSSPSEPGS SCSPTPPPVP241RRGTHTTVSQ VQPPPSKASA PEPPAEEEVA TGTTSASDDL EALGTLSLGT TEEKAAAEAA301VPRTIGAELM ELVRRNTGLS HELCRVAIGI IVGHIQASVP ASSPVMEQVL LSLVEGKDLS361MALPSGQVCH DQQRLEVIFA DLARRKDDAQ QRSWALYEDE GVIRCYLEEL LHILTDADPE421VCKKMCKRNE FESVLALVAY YQMEHRASLR LLLLKCFGAM CSLDAAIIST LVSSVLPVEL481ARDMQTDTQD HQKLCYSALI LAMVFSMGEA VPYAHYEHLG TPFAQFLLNI VEDGLPLDTT541EQLPDLCVNL LLALNLHLPA ADQNVIMAAL SKHANVKIFS EKLLLLLNRG DDPVRIFKHE601PQPPHSVLKF LQDVFGSPAT AAIFYHTDMM ALIDITVRHI ADLSPGDKLR MESLSLMHAI661VRTTPYLQHR HRLPDLQAIL RRILNEEETS PQCQMDRMIV REMCKEFLVL GEAPSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
715 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.