LSBio
Human NCKIPSD / AF3P21 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NCKIPSD / AF3P21 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NCKIPSD antibodies →
$153.00each
Catalog Number: LS-G90400-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NCKIPSD (NM_184231) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AF3P21 | Dia-interacting protein 1 | DIP-1 | DIP1 | Dia interacting protein | DIP | NCKIPSD | ORF1 | SH3 adapter protein SPIN90 | SPIN90 | WISH | WASLBP | VIP54
Origin Species
Human
NCBI GenBank
NM_016453
SwissProt
Q9NZQ3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYRALYAFRS AEPNALAFAA GETFLVLERS SAHWWLAARA RSGETGYVPP AYLRRLQGLE
61QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGPS ASSVAVMTSS
121TSDHHLDAAA ARQPNGVCRA GFERQHSLPS SEHLGADGGL YQIPPQPRRA APTTPPPPVK
181RRDREALMAS GSGGHNTMPS GGNSVSSGSS VSSTSLDTLY TSSSPSEPGS SCSPTPPPVP
241RRGTHTTVSQ VQPPPSKASA PEPPAEEEVA TGTTSASDDL EALGTLSLGT TEEKAAAEAA
301VPRTIGAELM ELVRRNTGLS HELCRVAIGI IVGHIQASVP ASSPVMEQVL LSLVEGKDLS
361MALPSGQVCH DQQRLEVIFA DLARRKDDAQ QRSWALYEDE GVIRCYLEEL LHILTDADPE
421VCKKMCKRNE FESVLALVAY YQMEHRASLR LLLLKCFGAM CSLDAAIIST LVSSVLPVEL
481ARDMQTDTQD HQKLCYSALI LAMVFSMGEA VPYAHYEHLG TPFAQFLLNI VEDGLPLDTT
541EQLPDLCVNL LLALNLHLPA ADQNVIMAAL SKHANVKIFS EKLLLLLNRG DDPVRIFKHE
601PQPPHSVLKF LQDVFGSPAT AAIFYHTDMM ALIDITVRHI ADLSPGDKLR MESLSLMHAI
661VRTTPYLQHR HRLPDLQAIL RRILNEEETS PQCQMDRMIV REMCKEFLVL GEAPS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.