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NCKAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NCKAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102829-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCKAP5 (NM_207481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
AB005217

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV
61AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRMEETVR
121NLLQSQGSPE QKKEETVNIM VYQEKLSEEE RKHKEALEDL HMVVDEDSRS ESSSTDEGKE
181KTKLLLERLK ALEAENSALA LENENQREQY ERCLDEVANQ VVQALLTQKD LREECVKLKT
241RVFDLEQQNR TLSILFQQRV RPTSDLLLQK LHSRLLDLSS GDLLSEVERN RSLTQSRTDA
301EVHEHQLNTK SALKCPGLGA VIPGHLCPRN SYSSSSELSL SSTCSEYSSG SSYTWHDGKN
361LRKRVKEANE NLQEDEDDAV ADSVFQSHII ESNCQMRTLD SGIGTFPLPD SGNRSTGRYL
421CQPDSPEDAE PLLPLQSALS AVSSMRAQTL EREVPSSTDG QRPADSAIVH STSDPIMTAR
481GMRPLQSRLP KPASSGKVSS QKQNEAEPRP QTCSSFGYAE DPMASQPLPD WGSEVAATGT
541QDKAPRMCTY SASGGSNSDS DLDYGDNGFG AGRGQLVKAL KSAAPEIETT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.