NCKAP5 Antibody (aa69-118)
Catalog Number: LS-C205525-100
100 µL (0.5 mg/mL) / $433.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NCKAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G79803-20
Catalog Number: LS-G79803
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NCKAP5 (NM_207481) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- AB005217
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV61AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRMEETVR121NLLQSQGSPE QKKEETVNIM VYQEKLSEEE RKHKEALEDL HMVVDEDSRS ESSSTDEGKE181KTKLLLERLK ALEAENSALA LENENQREQY ERCLDEVANQ VVQALLTQKD LREECVKLKT241RVFDLEQQNR TLSILFQQRV RPTSDLLLQK LHSRLLDLSS GDLLSEVERN RSLTQSRTDA301EVHEHQLNTK SALKCPGLGA VIPGHLCPRN SYSSSSELSL SSTCSEYSSG SSYTWHDGKN361LRKRVKEANE NLQEDEDDAV ADSVFQSHII ESNCQMRTLD SGIGTFPLPD SGNRSTGRYL421CQPDSPEDAE PLLPLQSALS AVSSMRAQTL EREVPSSTDG QRPADSAIVH STSDPIMTAR481GMRPLQSRLP KPASSGKVSS QKQNEAEPRP QTCSSFGYAE DPMASQPLPD WGSEVAATGT541QDKAPRMCTY SASGGSNSDS DLDYGDNGFG AGRGQLVKAL KSAAPEIETTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
590 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
