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NBPF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NBPF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NBPF15 antibodies →
$725.00each
Catalog Number: LS-G105259-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NBPF15(NBPF15) (NM_173638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AG3 | AB14 | NBPF16
Origin Species
Human
NCBI GenBank
NM_001170755

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVSAGPLSS EKAEMNILEI NEKLRPQLAE KKQQFRNLKE KCFLTQLAGF LANRQKKYKY
61EECKDLIKFM LRNERQFKEE KLAEQLKQAE ELRQYKVLVH AQERELTQLR EKLREGRDAS
121RSLNEHLQAL LTPDEPDKSQ GQDLQEQLAE GCRLTQHLVQ KLSPENDNDD DEDVQVEVAE
181KVQKSSAPRE MQKAEEKEVP EDSLEECAIT CSNSHGPYDS NQPHKKTKIT FEEDKVDSTL
241IGSSSHVEWE DAVHIIPENE SDDEEEEEKG PVSPRNLQES EEEEVPQESW DEGYSTLSIP
301PEMLASYQSY SSTFHSLEEQ QVCMAVDIGR HRWDQVKKED QEATGPRLSR ELLDEKEPEV
361LQDSLDRCYS TPSGCLELTD SCQPYRSAFY VLEQQRVGLA IDMDEIEKYQ EVEEDQDPSC
421PRLSRELLDE KEPEVLQDSL DRCYSTPSDY LELPDLGQPY SSAVYSLEEQ YLGLALAVDR
481IKKDQEEEED QGPPCPRLSR ELLEVVEPEV LQDSLDRCYS TPSSCLEQPD SCQPYGSSFY
541ALEEKHVGFS LDVGEIEKKG KGKKRRGRRS KKKRRRGRKE GEDDNPPCPR LYGVLMEVEE
601PEVLQDSLDR CYSTPSMYFE QPDSFQHYRS VFYSFEEEHI SFALYVDNRF FTLTVTSLHL
661VFQMGVIFPQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.