NBPF15 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G105259-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NBPF15 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95995-20
Catalog Number: LS-G95995
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NBPF15(NBPF15) (NM_173638) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AG3 | AB14 | NBPF16
- Origin Species
- Human
- NCBI GenBank
- NM_001170755
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVVSAGPLSS EKAEMNILEI NEKLRPQLAE KKQQFRNLKE KCFLTQLAGF LANRQKKYKY61EECKDLIKFM LRNERQFKEE KLAEQLKQAE ELRQYKVLVH AQERELTQLR EKLREGRDAS121RSLNEHLQAL LTPDEPDKSQ GQDLQEQLAE GCRLTQHLVQ KLSPENDNDD DEDVQVEVAE181KVQKSSAPRE MQKAEEKEVP EDSLEECAIT CSNSHGPYDS NQPHKKTKIT FEEDKVDSTL241IGSSSHVEWE DAVHIIPENE SDDEEEEEKG PVSPRNLQES EEEEVPQESW DEGYSTLSIP301PEMLASYQSY SSTFHSLEEQ QVCMAVDIGR HRWDQVKKED QEATGPRLSR ELLDEKEPEV361LQDSLDRCYS TPSGCLELTD SCQPYRSAFY VLEQQRVGLA IDMDEIEKYQ EVEEDQDPSC421PRLSRELLDE KEPEVLQDSL DRCYSTPSDY LELPDLGQPY SSAVYSLEEQ YLGLALAVDR481IKKDQEEEED QGPPCPRLSR ELLEVVEPEV LQDSLDRCYS TPSSCLEQPD SCQPYGSSFY541ALEEKHVGFS LDVGEIEKKG KGKKRRGRRS KKKRRRGRKE GEDDNPPCPR LYGVLMEVEE601PEVLQDSLDR CYSTPSMYFE QPDSFQHYRS VFYSFEEEHI SFALYVDNRF FTLTVTSLHL661VFQMGVIFPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
670 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
