Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MTNR1A / Melatonin Receptor 1a Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100690-100
Catalog Number: LS-G100690
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Melatonin Receptor 1A(MTNR1A) (NM_005958), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Mel-1a melatonin receptor | MEL-1A-R | Melatonin 1a receptor | Melatonin receptor 1A | MT1 melatonin receptor | MT1 receptor | Mel1a receptor | Melatonin receptor type 1A | MT1 | MTNR1A
- Origin Species
- Human
- NCBI GenBank
- NM_005958
- SwissProt
- P48039
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQGNGSALPN ASQPVLRGDG ARPSWLASAL ACVLIFTIVV DILGNLLVIL SVYRNKKLRN61AGNIFVVSLA VADLVVAIYP YPLVLMSIFN NGWNLGYLHC QVSGFLMGLS VIGSIFNITG121IAINRYCYIC HSLKYDKLYS SKNSLCYVLL IWLLTLAAVL PNLRAGTLQY DPRIYSCTFA181QSVSSAYTIA VVVFHFLVPM IIVIFCYLRI WILVLQVRQR VKPDRKPKLK PQDFRNFVTM241FVVFVLFAIC WAPLNFIGLA VASDPASMVP RIPEWLFVAS YYMAYFNSCL NAIIYGLLNQ301NFRKEYRRII VSLCTARVFF VDSSNDVADR VKWKPSPLMT NNNVVKVDSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
350 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.