LSBio
Human MTNR1A / Melatonin Receptor 1a Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MTNR1A / Melatonin Receptor 1a Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MTNR1A antibodies →
$153.00each
Catalog Number: LS-G77302-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Melatonin Receptor 1A(MTNR1A) (NM_005958) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Mel-1a melatonin receptor | MEL-1A-R | Melatonin 1a receptor | Melatonin receptor 1A | MT1 melatonin receptor | MT1 receptor | Mel1a receptor | Melatonin receptor type 1A | MT1 | MTNR1A
Origin Species
Human
NCBI GenBank
NM_005958
SwissProt
P48039

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQGNGSALPN ASQPVLRGDG ARPSWLASAL ACVLIFTIVV DILGNLLVIL SVYRNKKLRN
61AGNIFVVSLA VADLVVAIYP YPLVLMSIFN NGWNLGYLHC QVSGFLMGLS VIGSIFNITG
121IAINRYCYIC HSLKYDKLYS SKNSLCYVLL IWLLTLAAVL PNLRAGTLQY DPRIYSCTFA
181QSVSSAYTIA VVVFHFLVPM IIVIFCYLRI WILVLQVRQR VKPDRKPKLK PQDFRNFVTM
241FVVFVLFAIC WAPLNFIGLA VASDPASMVP RIPEWLFVAS YYMAYFNSCL NAIIYGLLNQ
301NFRKEYRRII VSLCTARVFF VDSSNDVADR VKWKPSPLMT NNNVVKVDSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

350 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.