Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MTBP / Mdm2-Binding Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110510-100
Catalog Number: LS-G110510
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTBP (NM_022045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Mdm2-binding protein | MTBP | MDM2BP | HMTBP
- Origin Species
- Human
- NCBI GenBank
- NM_022045
- SwissProt
- Q96DY7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRYLLLVIW GEGKFPSAAS REAEHGPEVS SGEGTENQPD FTAANVYHLL KRSISASINP61EDSTFPACSV GGIPGSKKWF FAVQAIYGFY QFCSSDWQEI HFDTEKDKIE DVLQTNIEEC121LGAVECFEEE DSNSRESLSL ADLYEEAAEN LHQLSDKLPA PGRAMVDIIL LLSDKDPPKL181KDYLPTVGAL KHLREWYSAK ITIAGNHCEI NCQKIAEYLS ANVVSLEDLR NVIDSKELWR241GKIQIWERKF GFEISFPEFC LKGVTLKNFS TSNLNTDFLA KKIIPSKDKN ILPKVFHYYG301PALEFVQMIK LSDLPSCYMS DIEFELGLTN STKQNSVLLL EQISSLCSKV GALFVLPCTI361SNILIPPPNQ LSSRKWKEYI AKKPKTISVP DVEVKGECSS YYLLLQGNGN RRCKATLIHS421ANQINGSFAL NLIHGKMKTK TEEAKLSFPF DLLSLPHFSG EQIVQREKQL ANVQVLALEE481CLKRRKLAKQ PETVSVAELK SLLVLTRKHF LDYFDAVIPK MILRKMDKIK TFNILNDFSP541VEPNSSSLME TNPLEWPERH VLQNLETFEK TKQKMRTGSL PHSSEQLLGH KEGPRDSITL601LDAKELLKYF TSDGLPIGDL QPLPIQKGEK TFVLTPELSP GKLQVLPFEK ASVCHYHGIE661YCLDDRKALE RDGGFSELQS RLIRYETQTT CTRESFPVPT VLSPLPSPVV SSDPGSVPDG721EVLQNELRTE VSRLKRRSKD LNCLYPRKRL VKSESSESLL SQTTGNSNHY HHHVTSRKPQ781TERSLPVTCP LVPIPSCETP KLATKTSSGQ KSMHESKTSR QIKESRSQKH TRILKEVVTE841TLKKHSITET HECFTACSQR LFEISKFYLK DLKTSRGLFE EMKKTANNNA VQVIDWVLEK901TSKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
904 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.