Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Western blot using anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).
Monoclonal Mouse anti-Human MTBP / Mdm2-Binding Protein Antibody (clone PHL-1, aa667-812, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C19034-100
Catalog Number: LS-C19034
For bulk orders or custom formulations:
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- Description
- Mdm2-Binding Protein antibody LS-C19034 is an unconjugated mouse monoclonal antibody to Mdm2-Binding Protein (MTBP) (aa667-812) from human. It is reactive with human and chimpanzee. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Mdm2-binding protein | MTBP | MDM2BP | HMTBP
- Recommended Dilution: ELISA
- 1:2000 - 1:20000
- Recommended Dilution: WB
- 1:200 - 1:2000
- UniProt Number
- Q96DY7
- NCBI GenBank
- NM_022045 | NP_071328.2
- SwissProt
- Q96DY7
Target
- Target
- Human MTBP / Mdm2-Binding Protein
- Antigen Species
- Human
- Epitope
- This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.
- Gene Name
- MTBP
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- PHL-1
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
