LSBio
MROH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MROH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MROH8 antibodies →
$504.00each
Catalog Number: LS-G106233-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf132(MROH8) (NM_152503), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf132 | DJ621N11.3 | DJ621N11.4 | MROH8 | C20orf131
Origin Species
Human
NCBI GenBank
R93639
SwissProt
Q9H579

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLSRRGRGS RLAGSRAATS NLPRATALSP KSAGRGALSI SHAQLLTRRR HSSEQVPPES
61EPRADFRSGK WLQEPATGDA RDSRQALRAR MSSKHRICSQ EEVVIPCAYD SDSESVDLEL
121SNLEIIKKGS SSIELTDLDI PDIPGLHCEP LSHSPRHLTQ QDPLSEAIVE KLIQSIQKVF
181NGELKGELEK LKFLGDLSSL SQALPYDETA KSFIHSHIAD IVHTLNVLVQ EERPHSLSSS
241MRQEVFVTIA DLSYQDVHLL LGSEDRAELF SLTIKSIITL PSVRTLTQIQ EIMPNGTCNT
301ECLYRQTFQA FSEMLQSLVV KDPHLENLDT IIKHLVPWLQ SVKDHERERA TASMAQVLKC
361LSKHLNLKLP LRFQRLGHLV ALMALLCGDP QEKVAEEAAE GIHSLLHITL RLKYITHDKK
421DQQNLKRALT KCREFLELHS SAAKCFYNCP FRIAQVFEGF LDSNELCQFI MTTFDTLKTL
481KHPCIQRSAG ELLLTLAKNT ESQFEKVPEI MGVICAQLSI ISQPRVRQQI INTVSLFISR
541PKYTDIVLSF LLCHPVPYNR HLAEVWRMLS VELPSTTWIL WRLLRKLQKC HNEPAQEKMA
601YVAVAATDAL YEVFLGNRLR AATFRLFPQL LMTLLIQIHH SIGLTMSDVD IPSGLYTEQE
661VPSEVTPLCF AMQAAKTLLL RTCCLQEFNI MEKNKGWALL GGKDGHLQGL FLLANALLER
721NQLLAQKVMY LLVPLLNRGN DKHKLTSAGF FVELLRSPVA KRLPSIYSVA RFKDWLQDGN
781HLFRILGLRG LYNLVGHQEM REDIKSLLPY IVDSLRETDE KIVLSAIQIL LQLVRTMDFT
841TLAAMMRTLF SLFGDVRSDV HRFSVTLFGA AIKSVKNPDK KSIENQVLDS LVPLLLYSQD
901ENDAVAEESR QVLTICAQFL KWKLPREVYS KDPWHIKPTE AGTICRFFEK KVQGEN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

956 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.