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MROH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MROH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102664-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf132(MROH8) (NM_213632), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf132 | DJ621N11.3 | DJ621N11.4 | MROH8 | C20orf131
Origin Species
Human
NCBI GenBank
R93639
SwissProt
Q9H579

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLSRRGRGS RLAGSRAATS NLPRATALSP KSAGRGALSI SHAQLLTRRR HSSEQVPPES
61EPRADFRSGK WLQEPATGDA RDSRQALRAR MSSKHRICSQ EEVVIPCAYD SDSESVDLEL
121SNLEIIKKGS SSIELTDLDI PDIPGLHCEP LSHSPRHLTQ QDPLSEAIVE KLIQSIQKVF
181NGELKGELEK LKFLGDLSSL SQALPYDETA KSFIHSHIAD IVHTLNVLVQ EERPHSLSSS
241MRQEVFVTIA DLSYQDVHLL LGSEDRAELF SLTIKSIITL PSVRTLTQIQ EIMPNGTCNT
301ECLYRQTFQA FSEMLQSLVV KDPHLENLDT IIKLPLRFQR LGHLVALMAL LCGDPQEKVA
361EEAAEGIHSL LHITLRLKYI THDKKDQQNL KRALTKCREF LELHSSAAKC FYNCPFRIAQ
421VFEGFLDSNE LCQFIMTTFD TLKNLKHPCI QRSAGELLLT LAKNTESQFE KVPEIMGVIC
481AQLSIISQPR VRQQIINTVS LFISRPKYTD IVLSFLLCHP VPYNRHLAEV WRMLSVELPS
541TTWILWRLLR KLQKCHNEPA QEKMAYVAVA VSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.