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MRGPRF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MRGPRF Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102297-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRGPRF (NM_145015), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 168 | G-protein coupled receptor 140 | G-protein coupled receptor 168 | GPR140 | GPR168 | MRGPRF | MRGF | Mas-related gene F protein | G protein-coupled receptor 140 | MAS-related GPR | member F | RTA
Origin Species
Human
NCBI GenBank
NM_145015
SwissProt
Q96AM1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGNCSWEAH PGNRNKMCPG LSEAPELYSR GFLTIEQIAM LPPPAVMNYI FLLLCLCGLV
61GNGLVLWFFG FSIKRNPFSI YFLHLASADV GYLFSKAVFS ILNTGGFLGT FADYIRSVCR
121VLGLCMFLTG VSLLPAVSAE RCASVIFPAW YWRRRPKRLS AVVCALLWVL SLLVTCLHNY
181FCVFLGRGAP GAACRHMDIF LGILLFLLCC PLMVLPCLAL ILHVECRARR RQRSAKLNHV
241ILAMVSVFLV SSIYLGIDWF LFWVFQIPAP FPEYVTDLCI CINSSAKPIV YFLAGRDKSQ
301RLWEPLRVVF QRALRDGAEL GEAGGSTPNT VTMEMQCPPG NAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

343 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.