LSBio
Human MRGPRF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MRGPRF Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MRGPRF antibodies →
$153.00each
Catalog Number: LS-G88106-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MRGPRF (NM_001098515) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 168 | G-protein coupled receptor 140 | G-protein coupled receptor 168 | GPR140 | GPR168 | MRGPRF | MRGF | Mas-related gene F protein | G protein-coupled receptor 140 | MAS-related GPR | member F | RTA
Origin Species
Human
NCBI GenBank
NM_145015
SwissProt
Q96AM1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGNCSWEAH PGNRNKMCPG LSEAPELYSR GFLTIEQIAM LPPPAVMNYI FLLLCLCGLV
61GNGLVLWFFG FSIKRNPFSI YFLHLASADV GYLFSKAVFS ILNTGGFLGT FADYIRSVCR
121VLGLCMFLTG VSLLPAVSAE RCASVIFPAW YWRRRPKRLS AVVCALLWVL SLLVTCLHNY
181FCVFLGRGAP GAACRHMDIF LGILLFLLCC PLMVLPCLAL ILHVECRARR RQRSAKLNHV
241ILAMVSVFLV SSIYLGIDWF LFWVFQIPAP FPEYVTDLCI CINSSAKPIV YFLAGRDKSQ
301RLWEPLRVVF QRALRDGAEL GEAGGSTPNT VTMEMQCPPG NAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

343 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.