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MMP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MMP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110447-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MMP27 (NM_022122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Matrix metallopeptidase 27 | MMP27 | MMP-27 | Matrix metalloprotease 27 | Matrix metalloproteinase 27 | Matrix metalloproteinase-27
Origin Species
Human
NCBI GenBank
NM_022122
SwissProt
Q9H306

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKRLLLLFLF FITFSSAFPL VRMTENEENM QLAQAYLNQF YSLEIEGNHL VQSKNRSLID
61DKIREMQAFF GLTVTGKLDS NTLEIMKTPR CGVPDVGQYG YTLPGWRKYN LTYRIINYTP
121DMARAAVDEA IQEGLEVWSK VTPLKFTKIS KGIADIMIAF RTRVHGRCPR YFDGPLGVLG
181HAFPPGPGLG GDTHFDEDEN WTKDGAGFNL FLVAAHEFGH ALGLSHSNDQ TALMFPNYVS
241LDPRKYPLSQ DDINGIQSIY GGLPKEPAKP KEPTIPHACD PDLTFDAITT FRREVMFFKG
301RHLWRIYYDI TDVEFELIAS FWPSLPADLQ AAYENPRDKI LVFKDENFWM IRGYAVLPDY
361PKSIHTLGFP GRVKKIDAAV CDKTTRKTYF FVGIWCWRFD EMTQTMDKGF PQRVVKHFPG
421ISIRVDAAFQ YKGFFFFSRG SKQFEYDIKT KNITRIMRTN TWFQCKEPKN SSFGFDINKE
481KAHSGGIKIL YHKSLSLFIF GIVHLLKNTS IYQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.