MMP27 Antibody (aa80-129)
Catalog Number: LS-C121092
2 sizes / from $341.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MMP27 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87593-20
Catalog Number: LS-G87593
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MMP27 (NM_022122) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Matrix metallopeptidase 27 | MMP27 | MMP-27 | Matrix metalloprotease 27 | Matrix metalloproteinase 27 | Matrix metalloproteinase-27
- Origin Species
- Human
- NCBI GenBank
- NM_022122
- SwissProt
- Q9H306
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRLLLLFLF FITFSSAFPL VRMTENEENM QLAQAYLNQF YSLEIEGNHL VQSKNRSLID61DKIREMQAFF GLTVTGKLDS NTLEIMKTPR CGVPDVGQYG YTLPGWRKYN LTYRIINYTP121DMARAAVDEA IQEGLEVWSK VTPLKFTKIS KGIADIMIAF RTRVHGRCPR YFDGPLGVLG181HAFPPGPGLG GDTHFDEDEN WTKDGAGFNL FLVAAHEFGH ALGLSHSNDQ TALMFPNYVS241LDPRKYPLSQ DDINGIQSIY GGLPKEPAKP KEPTIPHACD PDLTFDAITT FRREVMFFKG301RHLWRIYYDI TDVEFELIAS FWPSLPADLQ AAYENPRDKI LVFKDENFWM IRGYAVLPDY361PKSIHTLGFP GRVKKIDAAV CDKTTRKTYF FVGIWCWRFD EMTQTMDKGF PQRVVKHFPG421ISIRVDAAFQ YKGFFFFSRG SKQFEYDIKT KNITRIMRTN TWFQCKEPKN SSFGFDINKE481KAHSGGIKIL YHKSLSLFIF GIVHLLKNTS IYQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
