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MLZE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MLZE Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109234-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GSDMC (NM_031415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Gasdermin C | MLZE | Gasdermin-C
Origin Species
Human
NCBI GenBank
NM_031415
SwissProt
Q9BYG8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSMLERISK NLVKEIGSKD LTSVKYLLSA TKLRQFVILR KKKDSRSSFW EQSDYVPVEF
61SLNDILEPSS SVLETVVTGP FHFSDIMIQK HKADMGVNVG IEVSVSGEAS VDHGCSLEFQ
121IVTIPSPNLE DFQKRKLLDP EPSFLKECRR RGDNLYVVTE AVELINNTVL YDSSSVNILG
181KIALWITYGK GQGQGESLRV KKKALTLQKG MVMAYKRKQL VIKEKAILIS DDDEQRTFQD
241EYEISEMVGY CAARSEGLLP SFHTISPTLF NASSNDMKLK PELFLTQQFL SGHLPKYEQV
301HILPVGRIEE PFWQNFKHLQ EEVFQKIKTL AQLSKDVQDV MFYSILAMLR DRGALQDLMN
361MLELDSSGHL DGPGGAILKK LQQDSNHAWF NPKDPILYLL EAIMVLSDFQ HDLLACSMEK
421RILLQQQELV RSILEPNFRY PWSIPFTLKP ELLAPLQSEG LAITYGLLEE CGLRTELDNP
481RSTWDVEAKM PLSALYGTLS LLQQLAEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.