Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MICU3 / EFHA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104397-100
Catalog Number: LS-G104397
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFHA2(MICU3) (NM_181723), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EFHA2 | EF hand domain family A2
- Origin Species
- Human
- SwissProt
- Q86XE3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAALRRLLWP PPRVSPPLCA HQPLLGPWGR PAVTTLGLPG RPFSSREDEE RAVAEAAWRR61RRRWGELSVA AAAGGGLVGL VCYQLYGDPR AGSPATGRPS KSAATEPEDP PRGRGMLPIP121VAAAKETVAI GRTDIEDLDL YATSRERRFR LFASIECEGQ LFMTPYDFIL AVTTDEPKVA181KTWKSLSKQE LNQMLAETPP VWKGSSKLFR NLKEKGVISY TEYLFLLCIL TKPHAGFRIA241FNMFDTDGNE MVDKKEFLVL QEIFRKKNEK REIKGDEEKR AMLRLQLYGY HSPTNSVLKT301DAEELVSRSY WDTLRRNTSQ ALFSDLAERA DDITSLVTDT TLLVHFFGKK GKAELNFEDF361YRFMDNLQTE VLEIEFLSYS NGMNTISEED FAHILLRYTN VENTSVFLEN VRYSIPEEKG421ITFDEFRSFF QFLNNLEDFA IALNMYNFAS RSIGQDEFKR AVYVATGLKF SPHLVNTVFK481IFDVDKDDQL SYKEFIGIMK DRLHRGFRGY KTVQKYPTFK SCLKKELHSRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
530 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.