LSBio
Human MICU3 / EFHA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MICU3 / EFHA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MICU3 antibodies →
$153.00each
Catalog Number: LS-G93945-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EFHA2(MICU3) (NM_181723) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EFHA2 | EF hand domain family A2
Origin Species
Human
SwissProt
Q86XE3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAALRRLLWP PPRVSPPLCA HQPLLGPWGR PAVTTLGLPG RPFSSREDEE RAVAEAAWRR
61RRRWGELSVA AAAGGGLVGL VCYQLYGDPR AGSPATGRPS KSAATEPEDP PRGRGMLPIP
121VAAAKETVAI GRTDIEDLDL YATSRERRFR LFASIECEGQ LFMTPYDFIL AVTTDEPKVA
181KTWKSLSKQE LNQMLAETPP VWKGSSKLFR NLKEKGVISY TEYLFLLCIL TKPHAGFRIA
241FNMFDTDGNE MVDKKEFLVL QEIFRKKNEK REIKGDEEKR AMLRLQLYGY HSPTNSVLKT
301DAEELVSRSY WDTLRRNTSQ ALFSDLAERA DDITSLVTDT TLLVHFFGKK GKAELNFEDF
361YRFMDNLQTE VLEIEFLSYS NGMNTISEED FAHILLRYTN VENTSVFLEN VRYSIPEEKG
421ITFDEFRSFF QFLNNLEDFA IALNMYNFAS RSIGQDEFKR AVYVATGLKF SPHLVNTVFK
481IFDVDKDDQL SYKEFIGIMK DRLHRGFRGY KTVQKYPTFK SCLKKELHSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.