Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MCF2 / DBL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130342-100
Catalog Number: LS-G130342
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCF2 (NM_001171876), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DBL | Oncogene MCF2 (oncogene DBL) | Proto-oncogene MCF-2 | ARHGEF21 | MCF2 | Proto-oncogene DBL
- Origin Species
- Human
- NCBI GenBank
- NM_005369
- SwissProt
- P10911
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDIAFLSGG RGKDNAWIIT FPENCNFRCI PEEVIAKVLT YLTSIARQNG SDSRFTIILD61RRLDTWSSLK ISLQKISASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM121LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA181ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR241QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ301QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC361NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ421KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG481FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQVGVGYS FFQACKLFSK GKKTWRQNQS541NLKIEVVPDC QEKRSSGPSS SLDNGNSLDV LKNHVLNELI QTERVYVREL YTVLLGYRAE601MDNPEMFDLM PPLLRNKKDI LFGNMAEIYE FHNDIFLSSL ENCAHAPERV GPCFLERKDD661FQMYAKYCQN KPRSETIWRK YSECAFFQEC QRKLKHRLRL DSYLLKPVQR ITKYQLLLKE721LLKYSKDCEG SALLKKALDA MLDLLKSVND SMHQIAINGY IGNLNELGKM IMQGGFSVWI781GHKKGATKMK DLARFKPMQR HLFLYEKAIV FCKRRVESGE GSDRYPSYSF KHCWKMDEVG841ITEYVKGDNR KFEIWYGEKE EVYIVQASNV DVKMTWLKEI RNILLKQQEL LTVKKRKQQD901QLTERDKFQI SLQQNDEKQQ GAFISTEETE LEHTSTVVEV CEAIASVQAE ANTVWTEASQ961SAEISEEPAE WSSNYFYPTY DENEEENRPL MRPVSEMALL YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1001 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.