MCF2 / DBL Antibody
Catalog Number: LS-C747909
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MCF2 / DBL Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78426-20
Catalog Number: LS-G78426
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MCF2 (NM_001171876) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DBL | Oncogene MCF2 (oncogene DBL) | Proto-oncogene MCF-2 | ARHGEF21 | MCF2 | Proto-oncogene DBL
- Origin Species
- Human
- NCBI GenBank
- NM_005369
- SwissProt
- P10911
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDIAFLSGG RGKDNAWIIT FPENCNFRCI PEEVIAKVLT YLTSIARQNG SDSRFTIILD61RRLDTWSSLK ISLQKISASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM121LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA181ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR241QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ301QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC361NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ421KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG481FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQVGVGYS FFQACKLFSK GKKTWRQNQS541NLKIEVVPDC QEKRSSGPSS SLDNGNSLDV LKNHVLNELI QTERVYVREL YTVLLGYRAE601MDNPEMFDLM PPLLRNKKDI LFGNMAEIYE FHNDIFLSSL ENCAHAPERV GPCFLERKDD661FQMYAKYCQN KPRSETIWRK YSECAFFQEC QRKLKHRLRL DSYLLKPVQR ITKYQLLLKE721LLKYSKDCEG SALLKKALDA MLDLLKSVND SMHQIAINGY IGNLNELGKM IMQGGFSVWI781GHKKGATKMK DLARFKPMQR HLFLYEKAIV FCKRRVESGE GSDRYPSYSF KHCWKMDEVG841ITEYVKGDNR KFEIWYGEKE EVYIVQASNV DVKMTWLKEI RNILLKQQEL LTVKKRKQQD901QLTERDKFQI SLQQNDEKQQ GAFISTEETE LEHTSTVVEV CEAIASVQAE ANTVWTEASQ961SAEISEEPAE WSSNYFYPTY DENEEENRPL MRPVSEMALL YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1001 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
