LSBio
MARCO Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MARCO Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MARCO antibodies →
$504.00each
Catalog Number: LS-G115090-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MARCO (NM_006770), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MARCO | Macrophage receptor MARCO | Macrophage receptors marco | SCARA2
Origin Species
Human
NCBI GenBank
NM_006770
SwissProt
Q9UEW3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRNKKILKED ELLSETQQAA FHQIAMEPFE INVPKPKRRN GVNFSLAVVV IYLILLTAGA
61GLLVVQVLNL QARLRVLEMY FLNDTLAAED SPSFSLLQSA HPGEHLAQGA SRLQVLQAQL
121TWVRVSHEHL LQRVDNFTQN PGMFRIKGEQ GAPGLQGHKG AMGMPGAPGP PGPPAEKGAK
181GAMGRDGATG PSGPQGPPGV KGEAGLQGPQ GAPGKQGATG TPGPQGEKGS KGDGGLIGPK
241GETGTKGEKG DLGLPGSKGD RGMKGDAGVM GPPGAQGSKG DFGRPGPPGL AGFPGAKGDQ
301GQPGLQGVPG PPGAVGHPGA KGEPGSAGSP GRAGLPGSPG SPGATGLKGS KGDTGLQGQQ
361GRKGESGVPG PAGVKGEQGS PGLAGPKGAP GQAGQKGDQG VKGSSGEQGV KGEKGERGEN
421SVSVRIVGSS NRGRAEVYYS GTWGTICDDE WQNSDAIVFC RMLGYSKGRA LYKVGAGTGQ
481IWLDNVQCRG TESTLWSCTK NSWGHHDCSH EEDAGVECSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.