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MARCH10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MARCH10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118945-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF190(MARCH10) (NM_001100875), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MARCH-X | MARCH10 | Rnf190 | RING finger protein 190
Origin Species
Human
NCBI GenBank
NM_152598
SwissProt
Q8NA82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLHDARDRQK FFSDVQYLRD MQHKVDSEYQ ACLRRQEYRR DPNEKKRDQF WGQETSFERS
61RFSSRSSSKQ SSSEEDALTE PRSSIKISAF KCDSKLPAID QTSVKQKHKS TMTVRKAEKV
121DPSEPSPADQ APMVLLRKRK PNLRRFTVSP ESHSPRASGD RSRQKQQWPA KVPVPRGADQ
181VVQQEGLMCN TKLKRPNQER RNLVPSSQPM TENAPDRAKK GDPSAPSQSE LHPALSQAFQ
241GKNSPQVLSE FSGPPLTPTT VGGPRKASFR FRDEDFYSIL SLNSRRESDD TEEETQSEEC
301LWVGVRSPCS PSHHKRSRFG GTSTPQAKNK NFEENAENCR GHSSRRSEPS HGSLRISNAM
361EPATERPSAG QRLSQDPGLP DRESATEKDR GGSENAKKSP LSWDTKSEPR QEVGVNAENV
421WSDCISVEHR PGTHDSEGYW KDYLNSSQNS LDYFISGRPI SPRSSVNSSY NPPASFMHSA
481LRDDIPVDLS MSSTSVHSSD SEGNSGFHVC QPLSPIRNRT PFASAENHNY FPVNSAHEFA
541VREAEDTTLT SQPQGAPLYT DLLLNPQGNL SLVDSSSSSP SRMNSEGHLH VSGSLQENTP
601FTFFAVSHFP NQNDNGSRMA ASGFTDEKET SKIKADPEKL KKLQESLLEE DSEEEGDLCR
661ICQIAGGSPS NPLLEPCGCV GSLQFVHQEC LKKWLKVKIT SGADLGAVKT CEMCKQGLLV
721DLGDFNMIEF YQKHQQSQAQ NELMNSGLYL VLLLHLYEQR FAELMRLNHN QVERERLSRN
781YPQPRTEENE NSELGDGNEG SISQSQVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

808 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.