MARCH10 Antibody (C-Terminus)
Catalog Number: LS-C151232-100
100 µL (0.5 mg/mL) / $433.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MARCH10 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G94054-20
Catalog Number: LS-G94054
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RNF190(MARCH10) (NM_152598) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MARCH-X | MARCH10 | Rnf190 | RING finger protein 190
- Origin Species
- Human
- NCBI GenBank
- NM_152598
- SwissProt
- Q8NA82
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLHDARDRQK FFSDVQYLRD MQHKVDSEYQ ACLRRQEYRR DPNEKKRDQF WGQETSFERS61RFSSRSSSKQ SSSEEDALTE PRSSIKISAF KCDSKLPAID QTSVKQKHKS TMTVRKAEKV121DPSEPSPADQ APMVLLRKRK PNLRRFTVSP ESHSPRASGD RSRQKQQWPA KVPVPRGADQ181VVQQEGLMCN TKLKRPNQER RNLVPSSQPM TENAPDRAKK GDPSAPSQSE LHPALSQAFQ241EKNSPQVLSE FSGPPLTPTT VGGPRKASFR FRDEDFYSIL SLNSRRESDD TEEETQSEEC301LWVGVRSPCS PSHHKRSRFG GTSTPQAKNK NFEENAENCR GHSSRRSEPS HGSLRISNAM361EPATERPSAG QRLSQDPGLP DRESATEKDR GGSENAKKSP LSWDTKSEPR QEVGVNAENV421WSDCISVEHR PGTHDSEGYW KDYLNSSQNS LDYFISGRPI SPRSSVNSSY NPPASFMHSA481LRDDIPVDLS MSSTSVHSSD SEGNSGFHVC QPLSPIRNRT PFASAENHNY FPVNSAHEFA541VREAEDTTLT SQPQGAPLYT DLLLNPQGNL SLVDSSSSSP SRMNSEGHLH VSGSLQENTP601FTFFAVSHFP NQNDNGSRMA ASGFTDEKET SKIKADPEKL KKLQESLLEE DSEEEGDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
657 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
