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MAP7D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP7D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130154-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP7D2 (NM_001168467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001168465

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGFLKSDER QRLAKERREE REKCLAAREQ QILEKQKRAR LQYEKQMEER WRKLEEQRQR
61EDQKRAAVEE KRKQKLREEE ERLEAMMRRS LERTQQLELK KKYSWGAPLA IGPGGHDACD
121KLSTSTMSLP KPTEPPMNKR LSSSTVAISY SPDRAPLGPL NPSYKSSPTR NIEKKKATST
181STSGAGDVGK EALSGGEASL VEKVKRGQRT ATSLPVVNFG SPLRRCEFSG GIPKRPSSPV
241ISKTATKAYP QSPKTTKPPY PGSPVKYRLP ALSGQDMPKR KAEKEKSNKE REGTLAQQAA
301GPQGEEALEK HVVDKHASEK HAAAAGGKAE NSAALGKPTA GTTDAGEAAK ILAEKRRQAR
361LQKEQEEQER LEKEEQDRLE REELKRKAEE ERLRLEEEAR KQEEERKRQE EEKKKQEGEE
421KRKAGEEAKR KAEEELLLKE KQEQEKQEKA MIEKQKEAAE TKAREVAEQM RLEREQIMLQ
481IEQERLERKK RIDEIMKRTR KSDVSPQVKK EDPKVGVQPA VCVEKKTKLV VPNKMEINGL
541NTCQEVNGVD HAAPETYPQD IFSNGLKPAG GLIHLDALDG KSNSLDDSTE EVQSMDVSPV
601SKEELISIPE FSPVSEMIPG VSLDQNGTGN ARALQDLLDF TGPPTFPKRS SENLSLDDCN
661KNLIEGFNSP GQETPLNTFC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.