LSBio
MAP7D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP7D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAP7D2 antibodies →
$725.00each
Catalog Number: LS-G106000-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP7D2 (NM_152780), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001168465

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERGGGGSGT GSRPEGTARG TSLPGKIAEP GAVRTSQPNY RPQGMEGFLK SDERQRLAKE
61RREEREKCLA AREQQILEKQ KRARLQYEKQ MEERWRKLEE QRQREDQKRA AVEEKRKQKL
121REEEERLEAM MRRSLERTQQ LELKKKYSWG APLAIGPGGH DACDKLSTST MSLPKPTEPP
181MNKRLSSSTV AISYSPDRVF HVCPRLAPLG PLNPSYKSSP TRNIEKKKAT STSTSGAGDV
241GKEALSGGEA SLVEKVKRGQ RTATSLPVVN FGSPLRRCEF SGGIPKRPSS PVISKTATKA
301YPQSPKTTKP PYPGSPVKYR LPALSGQDMP KRKAEKEKSN KEREGTLAQQ AAGPQGEEAL
361EKHVVDKHAS EKHAAAAGGK AENSAALGKP TAGTTDAGEA AKILAEKRRQ ARLQKEQEEQ
421ERLEKEEQDR LEREELKRKA EEERLRLEEE ARKQEEERKR QEEEKKKQEG EEKRKAGEEA
481KRKAEEELLL KEKQEQEKQE KAMIEKQKEA AETKAREVAE QMRLEREQIM LQIEQERLER
541KKRIDEIMKR TRKSDVSPQV KKEDPKVGVQ PAVCVEKKTK LVVPNKMEIN GLNTCQEVNG
601VDHAAPETYP QDIFSNGLKP AGGLIHLDAL DGKSNSLDDS TEEVQSMDVS PVSKEELISI
661PEFSPVSEMI PGVSLDQNGT GNARALQDLL DFTGPPTFPK RSSENLSLDD CNKNLIEGFN
721SPGQETPLNT FC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

732 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.