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MAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAP7 antibodies →
$504.00each
Catalog Number: LS-G131075-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ensconsin(MAP7) (NM_001198617), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ensconsin | E-MAP-115 | MAP7 | EMAP115 | MAP-7
Origin Species
Human
NCBI GenBank
NM_003980
SwissProt
Q14244

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELGAGGDG HRGGDGAVRS ETAPDSYKVQ DKKNASSRPA SAISGQNNNH SGNKPDPPPV
61LRVDDRQRLA RERREEREKQ LDPDRRSVST MNLSKYVDPV ISKRLSSSSA TLLNSPDRAR
121RLQLSPWESS VVNRLLTPTH SFLARSKSTA ALSGEAASCS PIIMPYKAAH SRNSMDRPKL
181FVTPPEGSSR RRIIHGTASY KKERERENVL FLTSGTRRAV SPSNPKARQP ARSRLWLPSK
241SLPHLPGTPR PTSSLPPGSV KAAPAQVRPP SPGNIRPVKR EVKVEPEKKD PEKEPQKVAN
301EPSLKGRAPL VKVEEATVEE RTPAEPEVGP AAPAMAPAPA SAPAPASAPA PAPVPTPAMV
361SAPSSTVNAS ASVKTSAGTT DPEEATRLLA EKRRLAREQR EKEERERREQ EELERQKREE
421LAQRVAEERT TRREEESRRL EAEQAREKEE QLQRQAEERA LREREEAERA QRQKEEEARV
481REEAERVRQE REKHFQREEQ ERLERKKRLE EIMKRTRRTE ATDKKTSDQR NGDIAKGALT
541GGTEVSALPC TTNAPGNGKP VGSPHVVTSH QSKVTVESTP DLEKQPNENG VSVQNENFEE
601IINLPIGSKP SRLDVTNSES PEIPLNPILA FDDEGTLGPL PQVDGVQTQQ TAEVI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

655 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.