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MAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAP7 antibodies →
$504.00each
Catalog Number: LS-G116963-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ensconsin(MAP7) (NM_003980), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ensconsin | E-MAP-115 | MAP7 | EMAP115 | MAP-7
Origin Species
Human
NCBI GenBank
NM_003980
SwissProt
Q14244

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELGAGGDG HRGGDGAVRS ETAPDSYKVQ DKKNASSRPA SAISGQNNNH SGNKPDPPPV
61LRVDDRQRLA RERREEREKQ LAAREIVWLE REERARQHYE KHLEERKKRL EEQRQKEERR
121RAAVEEKRRQ RLEEDKERHE AVVRRTMERS QKPKQKHNRW SWGGSLHGSP SIHSADPDRR
181SVSTMNLSKY VDPVISKRLS SSSATLLNSP DRARRLQLSP WESSVVNRLL TPTHSFLARS
241KSTAALSGEA ASCSPIIMPY KAAHSRNSMD RPKLFVTPPE GSSRRRIIHG TASYKKERER
301ENVLFLTSGT RRAVSPSNPK ARQPARSRLW LPSKSLPHLP GTPRPTSSLP PGSVKAAPAQ
361VRPPSPGNIR PVKREVKVEP EKKDPEKEPQ KVANEPSLKG RAPLVKVEEA TVEERTPAEP
421EVGPAAPAMA PAPASAPAPA SAPAPAPVPT PAMVSAPSST VNASASVKTS AGTTDPEEAT
481RLLAEKRRLA REQREKEERE RREQEELERQ KREELAQRVA EERTTRREEE SRRLEAEQAR
541EKEEQLQRQA EERALREWEE AERAQRQKEE EARVREEAER VRQEREKHFQ REEQERLERK
601KRLEEIMKRT RRTEATDKKT SDQRNGDIAK GALTGGTEVS ALPCTTNAPG NGKPVGSPHV
661VTSHQSKVTV ESTPDLEKQP NENGVSVQNE NFEEIINLPI GSKPSRLDVT NSESPEIPLN
721PILAFDDEGT LGPLPQVDGV QTQQTAEVI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

749 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.