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MAP4K4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP4K4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106611-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP4K4 (NM_145686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HGK | HPK/GCK-like kinase HGK | KIAA0687 | Misshapen | NIK | Nck-interacting kinase | HPK/GCK-like kinase | Ste20 group protein kinase HGK | MAP4K4 | MEKKK 4 | MEKKK4 | Zc1 | FLH21957 | MEK kinase kinase 4
Origin Species
Human
NCBI GenBank
NM_145686
SwissProt
O95819

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
150.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE
61DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ LWLVMEFCGA GSITDLVKNT
121KGNTLKEDWI AYISREILRG LAHLHIHHVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR
181TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR
241ALFLIPRNPP PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI
301QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES TLRRDFLRLQ
361QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE QQKEQRRRLE EQQRREREAR
421RQQEREQRRR EQEEKRRLEE LERRRKEEEE RRRAEEEKRR VEREQEYIRR QLEEEQRHLE
481VLQQQLLQEQ AMLLECRWRE MEEHRQAERL QRQLQQEQAY LLSLQHDHRR PHPQHSQQPP
541PPQQERSKPS FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS
601ESFSNGNSES VHPALQRPAE PQVQWSHLAS LKNNVSPVSR SHSFSDPSPK FAHHHLRSQD
661PCPPSRSEVL SQSSDSKSEA PDPTQKAWSR SDSDEVPPRV PVRTTSRSPV LSRRDSPLQG
721SGQQNSQAGQ RNSTSSIEPR LLWERVEKLV PRPGSGSSSG SSNSGSQPGS HPGSQSGSGE
781RFRVRSSSKS EGSPSQRLEN AVKKPEDKKE VFRPLKPAGE VDLTALAKEL RAVEDVRPPH
841KVTDYSSSSE ESGTTDEEDD DVEQEGADES TSGPEDTRAA SSLNLSNGET ESVKTMIVHD
901DVESEPAMTP SKEGTLIVRQ TQSASSTLQK HKSSSSFTPF IDPRLLQISP SSGTTVTSVV
961GFSCDGMRPE AIRQDPTRKG SVVNVNPTNT RPQSDTPEIR KYKKRFNSEI LCAALWGVNL
1021LVGTESGLML LDRSGQGKVY PLINRRRFQQ MDVLEGLNVL VTISGKKDKL RVYYLSWLRN
1081KILHNDPEVE KKQGWTTVGD LEGCVHYKVV KYERIKFLVI ALKSSVEVYA WAPKPYHKFM
1141AFKSFGELVH KPLLVDLTVE EGQRLKVIYG SCAGFHAVDV DSGSVYDIYL PTHIQCSIKP
1201HAIIILPNTD GMELLVCYED EGVYVNTYGR ITKDVVLQWG EMPTSVAYIR SNQTMGWGEK
1261AIEIRSVETG HLDGVFMHKR AQRLKFLCER NDKVFFASVR SGGSSQVYFM TLGRTSLLSW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1320 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.