MAP4K4 Antibody
Catalog Number: LS-C416145
2 sizes / from $312.00
Antibody: Rabbit Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MAP4K4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82067-20
Catalog Number: LS-G82067
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAP4K4 (NM_145686) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HGK | HPK/GCK-like kinase HGK | KIAA0687 | Misshapen | NIK | Nck-interacting kinase | HPK/GCK-like kinase | Ste20 group protein kinase HGK | MAP4K4 | MEKKK 4 | MEKKK4 | Zc1 | FLH21957 | MEK kinase kinase 4
- Origin Species
- Human
- NCBI GenBank
- NM_145686
- SwissProt
- O95819
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 150.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE61DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ LWLVMEFCGA GSITDLVKNT121KGNTLKEDWI AYISREILRG LAHLHIHHVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR181TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR241ALFLIPRNPP PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI301QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES TLRRDFLRLQ361QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE QQKEQRRRLE EQQRREREAR421RQQEREQRRR EQEEKRRLEE LERRRKEEEE RRRAEEEKRR VEREQEYIRR QLEEEQRHLE481VLQQQLLQEQ AMLLECRWRE MEEHRQAERL QRQLQQEQAY LLSLQHDHRR PHPQHSQQPP541PPQQERSKPS FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS601ESFSNGNSES VHPALQRPAE PQVQWSHLAS LKNNVSPVSR SHSFSDPSPK FAHHHLRSQD661PCPPSRSEVL SQSSDSKSEA PDPTQKAWSR SDSDEVPPRV PVRTTSRSPV LSRRDSPLQG721SGQQNSQAGQ RNSTSSIEPR LLWERVEKLV PRPGSGSSSG SSNSGSQPGS HPGSQSGSGE781RFRVRSSSKS EGSPSQRLEN AVKKPEDKKE VFRPLKPAGE VDLTALAKEL RAVEDVRPPH841KVTDYSSSSE ESGTTDEEDD DVEQEGADES TSGPEDTRAA SSLNLSNGET ESVKTMIVHD901DVESEPAMTP SKEGTLIVRQ TQSASSTLQK HKSSSSFTPF IDPRLLQISP SSGTTVTSVV961GFSCDGMRPE AIRQDPTRKG SVVNVNPTNT RPQSDTPEIR KYKKRFNSEI LCAALWGVNL1021LVGTESGLML LDRSGQGKVY PLINRRRFQQ MDVLEGLNVL VTISGKKDKL RVYYLSWLRN1081KILHNDPEVE KKQGWTTVGD LEGCVHYKVV KYERIKFLVI ALKSSVEVYA WAPKPYHKFM1141AFKSFGELVH KPLLVDLTVE EGQRLKVIYG SCAGFHAVDV DSGSVYDIYL PTHIQCSIKP1201HAIIILPNTD GMELLVCYED EGVYVNTYGR ITKDVVLQWG EMPTSVAYIR SNQTMGWGEK1261AIEIRSVETG HLDGVFMHKR AQRLKFLCER NDKVFFASVR SGGSSQVYFM TLGRTSLLSWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1320 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
