Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP3K9 / MLK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108405-100
Catalog Number: LS-G108405
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K9(MAP3K9) (NM_033141), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAP3K9 | MEKK9 | MLK1 | Mixed lineage kinase 1 | PRKE1
- Origin Species
- Human
- NCBI GenBank
- AF251442
- SwissProt
- P80192
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPSRALLGC LASAAAAAPP GEDGAGAGAE EEEEEEEEAA AAVGPGELGC DAPLPYWTAV61FEYEAAGEDE LTLRLGDVVE VLSKDSQVSG DEGWWTGQLN QRVGIFPSNY VTPRSAFSSR121CQPGGEDPSC YPPIQLLEID FAELTLEEII GIGGFGKVYR AFWIGDEVAV KAARHDPDED181ISQTIENVRQ EAKLFAMLKH PNIIALRGVC LKEPNLCLVM EFARGGPLNR VLSGKRIPPD241ILVNWAVQIA RGMNYLHDEA IVPIIHRDLK SSNILILQKV ENGDLSNKIL KITDFGLARE301WHRTTKMSAA GTYAWMAPEV IRASMFSKGS DVWSYGVLLW ELLTGEVPFR GIDGLAVAYG361VAMNKLALPI PSTCPEPFAK LMEDCWNPDP HSRPSFTNIL DQLTTIEESG FFEMPKDSFH421CLQDNWKHEI QEMFDQLRAK EKELRTWEEE LTRAALQQKN QEELLRRREQ ELAEREIDIL481ERELNIIIHQ LCQEKPRVKK RKGKFRKSRL KLKDGNRISL PSDFQHKFTV QASPTMDKRK541SLINSRSSPP ASPTIIPRLR AIQLTPGESS KTWGRSSVVP KEEGEEEEKR APKKKGRTWG601PGTLGQKELA SGDEGSPQRR EKANGLSTPS ESPHFHLGLK SLVDGYKQWS SSAPNLVKGP661RSSPALPGFT SLMEMALLAA SWVVPIDIEE DEDSEGPGSG ESRLQHSPSQ SYLCIPFPRG721EDGDGPSSDG IHEEPTPVNS ATSTPQLTPT NSLKRGGAHH RRCEVALLGC GAVLAATGLG781FDLLEAGKCQ LLPLEEPEPP AREEKKRREG LFQRSSRPRR STSPPSRKLF KKEEPMLLLG841DPSASLTLLS LSSISECNST RSLLRSDSDE IVVYEMPVSP VEAPPLSPCT HNPLVNVRVE901RFKRDPNQSL TPTHVTLTTP SQPSSHRRTP SDGALKPETL LASRSPSSNG LSPSPGAGML961KTPSPSRDPG EFPRLPDPNV VFPPTPRRWN TQQDSTLERP KTLEFLPRPR PSANRQRLDP1021WWFVSPSHAR STSPANSSST ETPSNLDSCF ASSSSTVEER PGLPALLPFQ AGPLPPTERT1081LLDLDAEGQS QDSTVPLCRA ELNTHRPAPY EIQQEFWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1118 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.