LSBio
Human MAP3K9 / MLK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MAP3K9 / MLK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G77415-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K9(MAP3K9) (NM_033141) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAP3K9 | MEKK9 | MLK1 | Mixed lineage kinase 1 | PRKE1
Origin Species
Human
NCBI GenBank
AF251442
SwissProt
P80192

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPSRALLGC LASAAAAAPP GEDGAGAGAE EEEEEEEEAA AAVGPGELGC DAPLPYWTAV
61FEYEAAGEDE LTLRLGDVVE VLSKDSQVSG DEGWWTGQLN QRVGIFPSNY VTPRSAFSSR
121CQPGGEDPSC YPPIQLLEID FAELTLEEII GIGGFGKVYR AFWIGDEVAV KAARHDPDED
181ISQTIENVRQ EAKLFAMLKH PNIIALRGVC LKEPNLCLVM EFARGGPLNR VLSGKRIPPD
241ILVNWAVQIA RGMNYLHDEA IVPIIHRDLK SSNILILQKV ENGDLSNKIL KITDFGLARE
301WHRTTKMSAA GTYAWMAPEV IRASMFSKGS DVWSYGVLLW ELLTGEVPFR GIDGLAVAYG
361VAMNKLALPI PSTCPEPFAK LMEDCWNPDP HSRPSFTNIL DQLTTIEESG FFEMPKDSFH
421CLQDNWKHEI QEMFDQLRAK EKELRTWEEE LTRAALQQKN QEELLRRREQ ELAEREIDIL
481ERELNIIIHQ LCQEKPRVKK RKGKFRKSRL KLKDGNRISL PSDFQHKFTV QASPTMDKRK
541SLINSRSSPP ASPTIIPRLR AIQLTPGESS KTWGRSSVVP KEEGEEEEKR APKKKGRTWG
601PGTLGQKELA SGDEGSPQRR EKANGLSTPS ESPHFHLGLK SLVDGYKQWS SSAPNLVKGP
661RSSPALPGFT SLMEMALLAA SWVVPIDIEE DEDSEGPGSG ESRLQHSPSQ SYLCIPFPRG
721EDGDGPSSDG IHEEPTPVNS ATSTPQLTPT NSLKRGGAHH RRCEVALLGC GAVLAATGLG
781FDLLEAGKCQ LLPLEEPEPP AREEKKRREG LFQRSSRPRR STSPPSRKLF KKEEPMLLLG
841DPSASLTLLS LSSISECNST RSLLRSDSDE IVVYEMPVSP VEAPPLSPCT HNPLVNVRVE
901RFKRDPNQSL TPTHVTLTTP SQPSSHRRTP SDGALKPETL LASRSPSSNG LSPSPGAGML
961KTPSPSRDPG EFPRLPDPNV VFPPTPRRWN TQQDSTLERP KTLEFLPRPR PSANRQRLDP
1021WWFVSPSHAR STSPANSSST ETPSNLDSCF ASSSSTVEER PGLPALLPFQ AGPLPPTERT
1081LLDLDAEGQS QDSTVPLCRA ELNTHRPAPY EIQQEFWS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1118 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.