Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP3K15 / ASK3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99248-100
Catalog Number: LS-G99248
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K15 (NM_001001671), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASK3 | BA723P2.3 | MAP3K15 | MAPK/ERK kinase kinase 15 | MEK kinase 15 | MEKK 15
- NCBI GenBank
- SK681D
- SwissProt
- Q6ZN16
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MACLTHRNET DARMEFYSLF HKGNKAGVQW HDLGSLQPLP PRFKRFSCLS LQSSWDYSLS61KFDERCCFLY VHDNSDDFQI YFSTEEQCSR FFSLVKEMIT NTAGSTVELE GETDGDTLEY121EYDHDANGER VVLGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKYLK181HRNIVQYLGS VSENGYIKIF MEQVPGGSLS ALLRSKWGPM KEPTIKFYTK QILEGLKYLH241ENQIVHRDIK GDNVLVNTYS GVVKISDFGT SKRLAGVNPC TETFTGTLQY MAPEIIDQGP301RGYGAPADIW SLGCTIIEMA TSKPPFHELG EPQAAMFKVG MFKIHPEIPE ALSAEARAFI361LSCFEPDPHK RATTAELLRE GFLRQVNKGK KNRIAFKPSE GPRGVVLALP TQGEPMATSS421SEHGSVSPDS DAQPDALFER TRAPRHHLGH LLSVPDESSA LEDRGLASSP EDRDQGLFLL481RKDSERRAIL YKILWEEQNQ VASNLQECVA QSSEELHLSV GHIKQIIGIL RDFIRSPEHR541VMATTISKLK VDLDFDSSSI SQIHLVLFGF QDAVNKILRN HLIRPHWMFA MDNIIRRAVQ601AAVTILIPEL RAHFEPTCET EGVDKDMDEA EEGYPPATGP GQEAQPHQQH LSLQLGELRQ661ETNRLLEHLV EKEREYQNLL RQTLEQKTQE LYHLQLKLKS NCITENPAGP YGQRTDKELI721DWLRLQGADA KTIEKIVEEG YTLSDILNEI TKEDLRYLRL RGGLLCRLWS AVSQYRRAQE781ASETKDKATRTRPLEQKLISEEDLAANDILDYKDDDDKV
788 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.