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Human MAP3K15 / ASK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MAP3K15 / ASK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MAP3K15 antibodies →
$220.00each
Catalog Number: LS-G88670-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K15 (NM_001001671) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASK3 | BA723P2.3 | MAP3K15 | MAPK/ERK kinase kinase 15 | MEK kinase 15 | MEKK 15
Origin Species
Human
NCBI GenBank
SK681D
SwissProt
Q6ZN16

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACLTHRNET DARMEFYSLF HKGNKAGVQW HDLGSLQPLP PRFKRFSCLS LQSSWDYSLS
61KFDERCCFLY VHDNSDDFQI YFSTEEQCSR FFSLVKEMIT NTAGSTVELE GETDGDTLEY
121EYDHDANGER VVLGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKYLK
181HRNIVQYLGS VSENGYIKIF MEQVPGGSLS ALLRSKWGPM KEPTIKFYTK QILEGLKYLH
241ENQIVHRDIK GDNVLVNTYS GVVKISDFGT SKRLAGVNPC TETFTGTLQY MAPEIIDQGP
301RGYGAPADIW SLGCTIIEMA TSKPPFHELG EPQAAMFKVG MFKIHPEIPE ALSAEARAFI
361LSCFEPDPHK RATTAELLRE GFLRQVNKGK KNRIAFKPSE GPRGVVLALP TQGEPMATSS
421SEHGSVSPDS DAQPDALFER TRAPRHHLGH LLSVPDESSA LEDRGLASSP EDRDQGLFLL
481RKDSERRAIL YKILWEEQNQ VASNLQECVA QSSEELHLSV GHIKQIIGIL RDFIRSPEHR
541VMATTISKLK VDLDFDSSSI SQIHLVLFGF QDAVNKILRN HLIRPHWMFA MDNIIRRAVQ
601AAVTILIPEL RAHFEPTCET EGVDKDMDEA EEGYPPATGP GQEAQPHQQH LSLQLGELRQ
661ETNRLLEHLV EKEREYQNLL RQTLEQKTQE LYHLQLKLKS NCITENPAGP YGQRTDKELI
721DWLRLQGADA KTIEKIVEEG YTLSDILNEI TKEDLRYLRL RGGLLCRLWS AVSQYRRAQE
781ASETKDKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

788 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.