Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP2K4 / MKK4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99942-100
Catalog Number: LS-G99942
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEK4(MAP2K4) (NM_003010), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAP2K4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
- NCBI GenBank
- NM_003010
- SwissProt
- P45985
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPSPSGGG GSGGGRGSGT PGPVGSPAPG HPAVSSMQGK RKALKLNFAN PPFKSTARFT61LNPNPTGVQN PHIERLRTHS IESSGKLKIS PEQHWDFTAE DLKDLGEIGR GAYGSVNKMV121HKPSGQIMAV KRIRSTVDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL181MSTSFDKFYK YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS241GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL301ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKE361LLKHPFILMY EERAVEVACY VCKILDQMPA TPSSPMYVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.