MAP2K4 / MKK4 Antibody
Catalog Number: LS-C417540
2 sizes / from $312.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MAP2K4 / MKK4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76963-20
Catalog Number: LS-G76963
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MEK4(MAP2K4) (NM_003010) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAP2K4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
- Origin Species
- Human
- NCBI GenBank
- NM_003010
- SwissProt
- P45985
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPSPSGGG GSGGGRGSGT PGPVGSPAPG HPAVSSMQGK RKALKLNFAN PPFKSTARFT61LNPNPTGVQN PHIERLRTHS IESSGKLKIS PEQHWDFTAE DLKDLGEIGR GAYGSVNKMV121HKPSGQIMAV KRIRSTVDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL181MSTSFDKFYK YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS241GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL301ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKE361LLKHPFILMY EERAVEVACY VCKILDQMPA TPSSPMYVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
